采用高效液相色谱法直接测定大鼠粪便样本中的虎杖苷及其代谢产物。
Direct determination of polydatin and its metabolite in rat excrement samples by high-performance liquid chromatography.
作者信息
Zhang Wenting, Li Qing, Zhu Ming, Huang Qinwei, Jia Ying, Bi Kaishun
机构信息
School of Pharmacy, Shenyang Pharmaceutical University, PR China.
出版信息
Chem Pharm Bull (Tokyo). 2008 Nov;56(11):1592-5. doi: 10.1248/cpb.56.1592.
Simultaneous determination of polydatin and its metabolite in excrement samples using high-performance liquid chromatography (HPLC) with UV detection was accomplished. After extracted them by C18 solid phase extraction, the samples were separated on a reversed-phase column. Detection wave-lengths were set at 306 nm. The separation was carried out with a gradient elution. The mobile phase was acetonitrile-water (containing 0.1% formic acid) at a flow rate of 1.0 ml.min(-1). The identities of the peaks were accomplished by comparing retention times, UV and mass data with reference compounds under the same conditions. The standard curve was rectilinear in the range of 0.803-642.6 microg x ml(-1) (r=1.0000) for polydatin, 0.407-325.8 microg x ml(-1) (r=1.0000) for resveratrol. The recoveries of the markers listed above were 102.2% and 97.3%, respectively. The verified method can be used to determine the contents of two compounds in samples of stomach, small intestine, caecum, and large intestine (including excrement) of rats fed with polydatin. The analytical results demonstrated that the metabolism of polydatin is mainly processed in the intestines; polydatin can be transformed into resveratrol by de-sugaring process.
采用高效液相色谱（HPLC）-紫外检测法同时测定粪便样品中的白藜芦醇苷及其代谢产物。经C18固相萃取提取后，样品在反相柱上进行分离。检测波长设定为306 nm。采用梯度洗脱进行分离。流动相为乙腈-水（含0.1%甲酸），流速为1.0 ml·min⁻¹。通过在相同条件下将峰的保留时间、紫外和质谱数据与对照品进行比较来确定峰的身份。白藜芦醇苷的标准曲线在0.803 - 642.6 μg·ml⁻¹范围内呈线性（r = 1.0000），白藜芦醇的标准曲线在0.407 - 325.8 μg·ml⁻¹范围内呈线性（r = 1.0000）。上述标志物的回收率分别为102.2%和97.3%。该验证方法可用于测定喂食白藜芦醇苷的大鼠胃、小肠盲肠和大肠（包括粪便）样品中两种化合物的含量。分析结果表明，白藜芦醇苷的代谢主要在肠道中进行；白藜芦醇苷可通过去糖基化过程转化为白藜芦醇。
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