Gao Shouhong, Fan Guorong, Hong Zhanying, Yin Xueping, Yang Shaolin, Wu Yutian
School of Pharmacy, Second Military Medical University, Shanghai Key Laboratory for Pharmaceutical Metabolites Research, No. 325, Guohe Road, Shanghai 200433, PR China.
J Pharm Biomed Anal. 2006 Apr 11;41(1):240-5. doi: 10.1016/j.jpba.2005.08.027. Epub 2006 Feb 3.
A reversed-phase high-performance liquid chromatographic (RPHPLC) method has been developed for the determination of polydatin (PD) in rat plasma, bile, urine, feces and tissue homogenates using 2,3,5,4'-tetrahydroxychrysophenine-beta-d-glucoside as an internal standard. The sample pretreatment included deproteinization for plasma samples and a liquid-liquid extraction for bile, urine, feces and tissue homogenates. Separation was obtained on a C18 reversed-phase column with the mobile phase consisting of methanol and water (35:65 v/v). The flow rate was 1 ml/min and the effluent was monitored at 310 nm. The method showed good linearity over the concentration ranges employed for various matrices (r > 0.998). The quantification limits of PD in rat plasma, bile, urine, feces and tissue homogenates were 0.0251, 0.126, 0.025 microg/ml, 0.189 and 0.0378 microg/g, respectively. The accuracy and precision of the method were less than 12.0% for the various matrices. No interferences from endogenous substances were found. The method was successfully applied to study the pharmacokinetics of PD in rats after intravenous administration.
已开发出一种反相高效液相色谱（RPHPLC）法，以2,3,5,4'-四羟基苯并菲-β-D-葡萄糖苷为内标，用于测定大鼠血浆、胆汁、尿液、粪便和组织匀浆中的虎杖苷（PD）。样品预处理包括血浆样品的去蛋白处理以及胆汁、尿液、粪便和组织匀浆的液-液萃取。在C18反相柱上进行分离，流动相由甲醇和水（35:65 v/v）组成。流速为1 ml/min，在310 nm处监测流出物。该方法在所采用的各种基质浓度范围内显示出良好的线性（r > 0.998）。大鼠血浆、胆汁、尿液、粪便和组织匀浆中PD的定量限分别为0.0251、0.126、0.025 μg/ml、0.189和0.0378 μg/g。该方法在各种基质中的准确度和精密度均小于12.0%。未发现内源性物质的干扰。该方法已成功应用于研究大鼠静脉注射后PD的药代动力学。
