Fallon A M, Wyatt G R
Biochim Biophys Acta. 1977 Feb 9;480(2):428-41. doi: 10.1016/0005-2744(77)90035-3.
Exceptionally high levels of guanosine 3'-5'-cyclic monophosphate (cyclic GMP) in the accessory reproductive gland of the male house cricket, Acheta domesticus, led to an investigation of cyclic nucleotide phosphodiesterase (EC 3.1.4.--) as a possible regulatory enzyme. Cricket cyclic nucleotide phosphodiesterase activity with cyclic GMP or cyclic AMP as substrate had a pH optimum around 9.0, required Mg2+ or Mn2+ for maximal activity, and was inhibited by EDTA and methylxanthines. Cyclic GMP phosphodiesterase occurred mainly in the soluble fraction of homogenates of accessory glands or whole crickets, but cyclic AMP phosphodiesterase in the accessory gland was primarily particulate. Kinetic analysis indicated three forms of cyclic GMP phosphodiesterase, with Km values at 2.9 muM, 71 muM and 1.5 mM. Chromatography of whole cricket or accessory gland extracts on DEAE cellulose gave an initial peak having comparable activity with either cyclic GMP or cyclic AMP, and a second peak specific for cyclic AMP. There were no appreciable changes in the specific activity or kinetic properties of accessory gland cyclic GMP phosphodiesterase during a developmental period over which cyclic GMP levels rise more than 500-fold. Thus, the accumulation of cyclic GMP in the accessory gland is probably not associated with concomitant developmental modulation of phosphodiesterase activity.
雄性家蟋蟀（Acheta domesticus）附属性腺中鸟苷 3'-5'-环磷酸（环鸟苷酸）水平异常高，这引发了对环核苷酸磷酸二酯酶（EC 3.1.4.--）作为一种可能的调节酶的研究。以环鸟苷酸或环腺苷酸为底物时，蟋蟀环核苷酸磷酸二酯酶活性的最适pH约为9.0，最大活性需要Mg2+或Mn2+，并受到EDTA和甲基黄嘌呤的抑制。环鸟苷酸磷酸二酯酶主要存在于附属性腺或整个蟋蟀匀浆的可溶部分，但附属性腺中的环腺苷酸磷酸二酯酶主要存在于颗粒部分。动力学分析表明存在三种形式的环鸟苷酸磷酸二酯酶，其Km值分别为2.9 μM、71 μM和1.5 mM。将整个蟋蟀或附属性腺提取物在DEAE纤维素上进行色谱分析，得到一个初始峰，其对环鸟苷酸或环腺苷酸具有相当的活性，以及一个对环腺苷酸特异的第二个峰。在环鸟苷酸水平升高超过500倍的发育时期，附属性腺环鸟苷酸磷酸二酯酶的比活性或动力学性质没有明显变化。因此，附属性腺中环鸟苷酸的积累可能与磷酸二酯酶活性的伴随发育调节无关。
