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在酿酒酵母中产生的烟草硝酸还原酶蛋白的特性

Characteristics of Nicotiana tabacum nitrate reductase protein produced in Saccharomyces cerevisiae.

作者信息

Truong H N, Meyer C, Daniel-Vedele F

机构信息

Laboratoire de Biologie Cellulaire, I.N.R.A.--Versailles, France.

出版信息

Biochem J. 1991 Sep 1;278 ( Pt 2)(Pt 2):393-7. doi: 10.1042/bj2780393.

DOI:10.1042/bj2780393
PMID:1898332
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1151355/
Abstract

Tobacco nitrate reductase (NR) produced in yeast retains cytochrome c reductase activity, but not NR activity. Biochemical data suggest that the haem and FAD domains are functional, and that the molybdenum cofactor (MoCo) domain is inactive owing to the absence of MoCo in yeast. The native form of the produced NR is dimeric. Thus MoCo is not involved in NR dimerization in higher plants, contrary to current assumptions.

摘要

酵母中产生的烟草硝酸还原酶（NR）保留了细胞色素c还原酶活性，但没有硝酸还原酶活性。生化数据表明，血红素和黄素腺嘌呤二核苷酸（FAD）结构域具有功能，而钼辅因子（MoCo）结构域由于酵母中缺乏MoCo而无活性。所产生的NR的天然形式是二聚体。因此，与目前的假设相反，MoCo不参与高等植物中NR的二聚化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50a2/1151355/ef09d174c146/biochemj00152-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50a2/1151355/c48fe7f90402/biochemj00152-0087-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50a2/1151355/ef09d174c146/biochemj00152-0088-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50a2/1151355/c48fe7f90402/biochemj00152-0087-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50a2/1151355/ef09d174c146/biochemj00152-0088-a.jpg

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本文引用的文献

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