移植到碘酸钠注射大鼠视网膜下间隙的大鼠间充质干细胞的分化
Differentiation of rat mesenchymal stem cells transplanted into the subretinal space of sodium iodate-injected rats.
作者信息
Gong Lihua, Wu Qiang, Song Beiwen, Lu Bin, Zhang Yi
机构信息
Department of Ophthalmology, Affiliated Sixth People's Hospital Shanghai Jiaotong University, Shanghai, China.
出版信息
Clin Exp Ophthalmol. 2008 Oct;36(7):666-71. doi: 10.1111/j.1442-9071.2008.01857.x.
PURPOSE
The differentiation of rat bone marrow mesenchymal stem cells (MSCs) was investigated in a retinal pigment epithelium (RPE) damage model induced by the administration of sodium iodate.
METHODS
Cultured rat MSCs were transfected with enhanced green fluorescent protein and transplanted into the subretinal space of rats injected 4 days earlier with sodium iodate. Immunofluorescence analysis was performed 5 weeks later.
RESULTS
The transduction efficiency was 99.9%. Viable MSCs were detected 5 weeks after transplantation, mainly in the subretinal space. The cells expressed pan-cytokeratin, glial fibrillary acidic protein and rhodopsin.
CONCLUSIONS
Bone marrow MSCs transplanted into the subretinal space of sodium iodate-injected rats have the ability to differentiate into RPE, photoreceptor and glial lineage cells.
目的
在由碘酸钠给药诱导的视网膜色素上皮（RPE）损伤模型中研究大鼠骨髓间充质干细胞（MSCs）的分化情况。
方法
将培养的大鼠MSCs用增强型绿色荧光蛋白转染，并移植到4天前注射了碘酸钠的大鼠的视网膜下间隙。5周后进行免疫荧光分析。
结果
转导效率为99.9%。移植5周后检测到存活的MSCs，主要位于视网膜下间隙。这些细胞表达泛细胞角蛋白、胶质纤维酸性蛋白和视紫红质。
结论
移植到注射了碘酸钠的大鼠视网膜下间隙的骨髓MSCs具有分化为RPE、光感受器和神经胶质谱系细胞的能力。
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