染色体失衡与增殖增加相关，可能导致胆脂瘤中的骨质破坏。
Chromosomal imbalances are associated with increased proliferation and might contribute to bone destruction in cholesteatoma.
作者信息
Ecsedi Szilvia, Rákosy Zsuzsa, Vízkeleti Laura, Juhász Attila, Sziklai István, Adány Róza, Balázs Margit
机构信息
Department of Preventive Medicine, Faculty of Public Health, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
出版信息
Otolaryngol Head Neck Surg. 2008 Nov;139(5):635-40. doi: 10.1016/j.otohns.2008.07.019.
OBJECTIVES
Our aim was to evaluate the copy number alterations of chromosomes 3, 7, 8, and 17 in middle ear cholesteatomas and define the association between the rate of cell proliferation and chromosome number changes.
METHODS
Tissues were obtained from 16 patients. Fluorescence in situ hybridization was performed on tumor imprint preparations. Cell proliferation was characterized with Ki-67 monoclonal antibody on cholesteatoma samples and on postauricular skins as control.
RESULTS
Different degrees of aneusomy were found for all chromosomes except for chromosome 3. Chromosome copy number alterations were associated with elevated proliferative rate and related also with the aggressiveness of the lesions.
CONCLUSIONS
Based on our results, we assume that aneusomy of chromosomes 7, 8, and 17 might play an important role during invasion of the adjacent bony structures of cholesteatoma, as well as associate with increased cell proliferation activity, which might lead to the aggressive behavior of the tissue.
目的
我们的目的是评估中耳胆脂瘤中3号、7号、8号和17号染色体的拷贝数改变，并确定细胞增殖率与染色体数目变化之间的关联。
方法
从16例患者身上获取组织。在肿瘤压片标本上进行荧光原位杂交。用Ki-67单克隆抗体对胆脂瘤样本及作为对照的耳后皮肤进行细胞增殖特征分析。
结果
除3号染色体外，所有染色体均发现不同程度的非整倍体。染色体拷贝数改变与增殖率升高相关，也与病变的侵袭性有关。
结论
基于我们的结果，我们推测7号、8号和17号染色体的非整倍体可能在胆脂瘤侵袭相邻骨质结构过程中起重要作用，并且与细胞增殖活性增加相关，这可能导致组织的侵袭性行为。
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