Chauhan Ranjit, Kazim Syed-Naqui, Kumar Manoj, Bhattacharjee Jayashree, Krishnamoorthy Narayanasamy, Sarin Shiv-Kumar
Department of Gastroenterology, G. B. Pant Hospital, Delhi University Affiliated, Room No. 201, Academic Block, New Delhi 110002, India.
World J Gastroenterol. 2008 Oct 28;14(40):6228-36. doi: 10.3748/wjg.14.6228.
To confirm the presence of recombination, full-length hepatitis B virus (HBV) from chronic patients was sequenced and analyzed.
Full-length HBV genomes from 12 patients were amplified and sequenced in an automated sequencer. Phylogenetic analysis was carried out on full-length, Core and preS2/Surface regions using MEGA software. SimPlot Boot Scanning and amino acid sequence analysis were performed for confirmation of recombination.
Eight patients were infected with genotype D strain; one patient with genotype A and three patients had genotype A and D recombination; two of them had cirrhosis and one had hepatocellular carcinoma. Phylogenetic analysis of core and preS2/surface regions separately showed evidence of genotype A and D recombination. The breakpoints of recombination were found to be at the start of preS2 and at the end of surface coding regions.
We identified and characterized recombinant A and D genotype HBV in hepatitis B surface antigen (HBsAg)-positive patients.
为证实重组的存在,对慢性患者的全长乙型肝炎病毒(HBV)进行测序和分析。
对12例患者的全长HBV基因组进行扩增,并在自动测序仪上测序。使用MEGA软件对全长、核心区和前S2/表面区进行系统发育分析。进行SimPlot Boot扫描和氨基酸序列分析以确认重组。
8例患者感染D基因型毒株;1例患者感染A基因型,3例患者存在A和D基因型重组;其中2例有肝硬化,1例有肝细胞癌。对核心区和前S2/表面区分别进行系统发育分析显示存在A和D基因型重组的证据。重组断点位于前S2起始处和表面编码区末端。
我们在乙型肝炎表面抗原(HBsAg)阳性患者中鉴定并表征了重组A和D基因型HBV。