Qian Zhao, Jianqiong Wang, Hongmei Li, Rong Zeng, Li Li, Jinping Zhang, Tao Shen
Kunming Medical College, Kunming, Yunnan, China ; Institute of Basic and Clinical Medicine, Provincial Key Laboratory for Birth Defects and Genetic Diseases, the First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming Science and Technology University, Kunming, Yunnan Province, China.
Clinical Laboratory, the First People's Hospital of Yunnan Province, Kunming, Yunnan Province, China.
Hepat Mon. 2015 May 23;15(5):e24422. doi: 10.5812/hepatmon.15(5)2015.24422. eCollection 2015 May.
The number of cases of HBV infection reported by the WHO for each district and country is positively correlated with the number of HBV sequences in the database isolated from the corresponding district and country.
This study determined distribution characteristics of HBV genotypes and subtypes in 14 countries neighboring China. The progress made in genomic research involving HBV was also reviewed.
Nine hundred fifty-one complete genome sequences of HBV from 14 countries neighboring China were selected from NCBI. The sequence-related information was analyzed and recorded. One hundred seventy-two sequences of HBV genotype B were screened for alignment using DNA star and MEGA 5.1.
Dominant HBV genotypes in the countries neighboring China were genotypes B, C and D and dominant subtypes were adw2 and adrq+. The association between genotype and serotype of HBV in these countries was shown to differ from previous research results. As shown by sequence alignment, the sequence divergence between five subgenotypes (B3, B5, B7, B8 and B9) was below 4%. The B subgenotypes shared six common specific amino acid sites in the S region.
The B3, B5, B7, B8 and B9 subgenotypes can be clustered into quasi-sub-genotype B3 and the open reading frame of HBV has a start codon preference; however, whether a mutation in the start codon in the pre-S2 region has an impact on survival and replication of HBV remains to be determined.
世界卫生组织报告的每个地区和国家的乙肝病毒（HBV）感染病例数与数据库中从相应地区和国家分离出的HBV序列数量呈正相关。
本研究确定了中国周边14个国家HBV基因型和亚型的分布特征。还综述了HBV基因组研究取得的进展。
从美国国立生物技术信息中心（NCBI）选取了中国周边14个国家的951条HBV完整基因组序列。对序列相关信息进行分析并记录。筛选出172条HBV B基因型序列，使用DNA star和MEGA 5.1进行比对。
中国周边国家的优势HBV基因型为B、C和D基因型，优势亚型为adw2和adrq +。这些国家中HBV基因型与血清型之间的关联与先前的研究结果不同。序列比对显示，5个亚基因型（B3、B5、B7、B8和B9）之间的序列差异低于4%。B亚基因型在S区共有6个共同的特异性氨基酸位点。
B3、B5、B7、B8和B9亚基因型可聚类为准亚基因型B3，且HBV的开放阅读框存在起始密码子偏好；然而，前S2区起始密码子的突变是否对HBV的生存和复制有影响仍有待确定。
