Visceral leishmaniasis caused by Leishmania infantum is an endemic zoonosis, present in the Mediterranean area and well recognized in Israel and Palestine for human and dog disease. A serological study using an ELISA technique was performed on 104 cats living in the Jerusalem area. Seroprevalence was 6.7% (7/104). Significant correlation between seropositive cat results and altitude > 2500 ft was observed (p = 0.02). This is the first serological survey of feline leishmaniasis (FL) in the Middle East. To prove cat involvement as a secondary host, more investigations are still needed. The study concludes that cat involvement in Leishmania host studies should not be ignored.