Marulappa Shashidhara Y, Kapil Sanjay
Oklahoma Animal Disease Diagnostic Laboratory, Center for Veterinary Health Sciences, Stillwater, Oklahoma 74078, USA
Clin Vaccine Immunol. 2009 Jan;16(1):127-31. doi: 10.1128/CVI.00304-08. Epub 2008 Nov 5.
Canine parvovirus (CPV) is the number one viral cause of enteritis, morbidity, and mortality in 8-week-old young puppies. We have developed twin assays (slide agglutination test [SAT] for CPV antigen and slide inhibition test [SIT] for CPV antibody) that are sensitive, specific, cost-effective, generic for all genotypes of CPV, and provide instant results for CPV antigen detection in feces and antibody quantification in serum. We found these assays to be useful for routine applications in kennels with large numbers of puppies at risk. The results of these assays are available in 1 min and do not require any special instrumentation. SAT-SIT technology will find applications in rapid screening of samples for other hemagglutinating emerging viruses of animals and humans (influenza virus and severe acute respiratory syndrome coronavirus).
犬细小病毒（CPV）是8周龄幼犬肠炎、发病和死亡的首要病毒病因。我们开发了双检测法（用于CPV抗原的玻片凝集试验[SAT]和用于CPV抗体的玻片抑制试验[SIT]），这些检测法灵敏、特异、性价比高，对所有CPV基因型通用，能即时检测粪便中的CPV抗原并定量血清中的抗体。我们发现这些检测法在有大量幼犬面临风险的犬舍常规应用中很有用。这些检测法的结果在1分钟内即可获得，且不需要任何特殊仪器。SAT-SIT技术将在快速筛查动物和人类其他血凝性新兴病毒（流感病毒和严重急性呼吸综合征冠状病毒）的样本中得到应用。
