Department of Veterinary Public Health, Faculty of Veterinary Medicine, Strada per Casamassima km 3, 70010 Valenzano (Bari), Italy.
Vet J. 2010 Jun;184(3):373-5. doi: 10.1016/j.tvjl.2009.04.006. Epub 2009 May 1.
Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a, n=51; CPV-2b, n=50; CPV-2c, n=100), containing CPV DNA loads >10(5) DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods.
犬细小病毒（CPV）感染的诊断通常通过快速免疫层析检测法进行，但这些检测方法是否能够检测到所有 CPV 变异株，包括最近发现的 CPV-2c，仍存在争议。为了确定这些检测方法是否能够检测到不同的 CPV 变异株，我们使用一种市售的内部检测方法对 201 份 CPV PCR 阳性粪便样本或直肠拭子进行了检测。根据实时 PCR 检测结果，从先前的研究中选择了含有 CPV DNA 载量>10(5)DNA 拷贝/mg 粪便的 CPV-2a（n=51）、CPV-2b（n=50）和 CPV-2c（n=100）样本。内部检测法对 CPV-2a、CPV-2b 和 CPV-2c 的阳性检出率分别为 80.4%、78.0%和 77.0%，证实了该检测方法能够检测到新型 CPV-2c 变异株。然而，考虑到之前观察到的内部检测方法的灵敏度限制，内部检测试剂盒的阴性结果应通过基于 PCR 的方法进行确认。
