Soler Laura, Martínez-Subiela Silvia, Tecles Fernando, Gutierrez Ana, Fuentes Pablo, Cerón Jose Joaquín
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Murcia, 30100 Espinardo Campus, Murcia, Spain.
J Vet Diagn Invest. 2008 Nov;20(6):799-803. doi: 10.1177/104063870802000615.
The potential use of total sialic acid (TSA) as an acute-phase biomarker in pigs was investigated through clinical and analytic validation of an automated commercial enzymatic-colorimetric assay. Intra- and interassay precision, accuracy, and detection limit were determined for the analytical validation of the method. The intra- and interassay coefficients of variation (%CV) were 2.27% and 3.99%, respectively, whereas the linearity under dilution calculation resulted in a coefficients of correlation of r = 0.995 and r = 0.999 for TSA high- and low-content serum samples, respectively, which indicated that the automated method was reliable to measure TSA in porcine serum samples. Average serum TSA concentrations in diseased pigs (median: 1779.16 mg/l; 5th percentile: 556.62 mg/l; 95th percentile: 963.06 mg/l) were 2.5-fold higher compared with healthy animals (median: 692.78 mg/l; 5th percentile: 1278.74 mg/l; 95th percentile: 2536.12 mg/l), which indicated that TSA can be considered as a moderate acute phase marker in swine. Therefore, TSA may be useful for detecting inflammatory conditions and could be included in biochemical profiles used to monitor the general status of pigs as a moderate acute phase marker.
通过对一种自动化商业酶比色测定法进行临床和分析验证，研究了总唾液酸（TSA）作为猪急性期生物标志物的潜在用途。为了对该方法进行分析验证，测定了批内和批间精密度、准确度和检测限。批内和批间变异系数（%CV）分别为2.27%和3.99%，而稀释计算下的线性关系显示，TSA高含量和低含量血清样本的相关系数分别为r = 0.995和r = 0.999，这表明该自动化方法可可靠地测量猪血清样本中的TSA。患病猪的血清TSA平均浓度（中位数：1779.16 mg/l；第5百分位数：556.62 mg/l；第95百分位数：963.06 mg/l）比健康动物（中位数：692.78 mg/l；第5百分位数：1278.74 mg/l；第95百分位数：2536.12 mg/l）高2.5倍，这表明TSA可被视为猪的中度急性期标志物。因此，TSA可能有助于检测炎症状态，并且作为中度急性期标志物可纳入用于监测猪总体健康状况的生化指标中。
