Dellgren C, Gad H H, Hamming O J, Melchjorsen J, Hartmann R
Department of Molecular Biology, Centre for Structural Biology, University of Aarhus, Aarhus C, Denmark.
Genes Immun. 2009 Mar;10(2):125-31. doi: 10.1038/gene.2008.87. Epub 2008 Nov 6.
Type III interferon (IFN) or IFN-lambda is a recently discovered family of IFNs that signal through the same downstream transcription factors as type I IFN but use a separate receptor complex composed of the IL-10R2 and the unique IFN-lambdaR1 receptor chains. We have established a simple and efficient expression system to produce highly pure and active IFN-lambda of the three human IFN-lambda isoforms (IFN-lambda1, -lambda2 and -lambda3) and used this to compare the biological activity of the different IFN-lambda subtypes. Surprisingly, we found IFN-lambda3 to possess the highest specific activity of the human IFN-lambda subtypes, exhibiting a twofold higher activity than IFN-lambda1 and a 16-fold higher activity than IFN-lambda2. Furthermore, in comparison with the commercially available preparations of IFN-lambda1 and -lambda2, we found our IFN-lambda preparation to be superior in activity.
III型干扰素（IFN）或λ干扰素是最近发现的一类干扰素，其通过与I型干扰素相同的下游转录因子进行信号传导，但使用由IL-10R2和独特的λ干扰素受体链组成的单独受体复合物。我们建立了一个简单高效的表达系统，以生产三种人λ干扰素亚型（λ干扰素1、λ干扰素2和λ干扰素3）的高纯度活性λ干扰素，并以此比较不同λ干扰素亚型的生物学活性。令人惊讶的是，我们发现λ干扰素3具有人λ干扰素亚型中最高的比活性，其活性比λ干扰素1高两倍，比λ干扰素2高16倍。此外，与市售的λ干扰素1和λ干扰素2制剂相比，我们发现我们制备的λ干扰素在活性方面更优。
