Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Antiviral Res. 2010 Dec;88(3):329-33. doi: 10.1016/j.antiviral.2010.10.005. Epub 2010 Oct 20.
Interferons lambda (IFN-λ) are the most recently defined members of the class III cytokine family. To investigate whether IFN-λ2 and IFN-λ3 displayed antiviral activity against influenza A virus (IAV), a number of cell lines induced with IFNs - as well as two established cell lines (A549-IFN-λ2 and A549-IFN-λ3) - were infected with IAV. Our results indicate that IFN-λ2 has statistically significant antiviral activity in A549-IFN-λ2 (P=0.0028) although less so than IFN-λ3, which reduced viral titer to 10% (P<0.0001). The reverse was observed for cells treated with IFNs, with IFN-λ2-treated A549 cells inhibiting IAV infection more efficiently than IFN-λ3-treated A549 cells. The antiviral effect on IFN-stimulated cells was most apparent on Vero cells (compared with MDCK and HeLa). Both IFNs significantly inhibited IAV replication and inhibition was observed in a dose-dependent manner, with an optimal IFN concentration of 20 ng/ml. IFN-λ2 was more potent than IFN-λ3 on Vero cells while IFN-λ3 appeared more efficient than IFN-λ2 on MDCK and HeLa cells.
干扰素 λ（IFN-λ）是 III 类细胞因子家族中最新定义的成员。为了研究 IFN-λ2 和 IFN-λ3 是否对甲型流感病毒（IAV）具有抗病毒活性，用 IFNs 诱导了许多细胞系以及两种已建立的细胞系（A549-IFN-λ2 和 A549-IFN-λ3）来感染 IAV。我们的结果表明，IFN-λ2 在 A549-IFN-λ2 中具有统计学上显著的抗病毒活性（P=0.0028），尽管不如 IFN-λ3 显著，IFN-λ3 将病毒滴度降低至 10%（P<0.0001）。用 IFNs 处理的细胞则相反，IFN-λ2 处理的 A549 细胞比 IFN-λ3 处理的 A549 细胞更有效地抑制 IAV 感染。IFN-λ2 在 Vero 细胞（与 MDCK 和 HeLa 相比）上对 IFN 刺激细胞的抗病毒作用最为明显。两种 IFN 均能显著抑制 IAV 复制，抑制作用呈剂量依赖性，最佳 IFN 浓度为 20ng/ml。IFN-λ2 在 Vero 细胞上比 IFN-λ3 更有效，而 IFN-λ3 在 MDCK 和 HeLa 细胞上比 IFN-λ2 更有效。
