Sudheer Pamidimarri D V N, Chattopadhyay Balaji, Reddy Muppala P
Discipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute, Council for Scientific and Industrial Research, G. B. Marg, Bhavnagar, Gujarat, India.
Mol Biol Rep. 2009 Sep;36(7):1929-35. doi: 10.1007/s11033-008-9401-6. Epub 2008 Nov 6.
The genus Jatropha belongs to the family Euphorbiaceae having significant economic importance. The present investigation was undertaken with an aim to understand phylogenetic relationships among seven species (J. curcas, J. glandulifera, J. gossypifolia, J. integerrima, J. multifida, J. podagrica, and J. tanjorensis.) which are widely distributed in India, using nuclear ribosomal DNA ITS sequence (nrDNA ITS) and to compare the results with multilocus marker analysis systems reported earlier for the same genus. The size variation obtained among sequenced nrDNA ITS regions was narrow and ranged from 647 to 654 bp. The overall mean genetic distance (GD) of genus Jatropha was found to be 0.385. Highest interspecific GD (0.419) was found between J. glandulifera and J. multifida. The least interspecific GD (0.085) was found between J. gossypifolia and J. tanjorensis. The highest intraspecific GD was observed in J. podagrica (0.011) and least in J. gossypifolia (0.002). The phylogram obtained using nrDNA ITS sequence showed congruence with the phylograms obtained using multilocus markers system reported earlier with minor variations. The present study also strongly supports high phylogenetic closeness of J. curcas and J. integerrima. The only exception found was J. podagrica which clustered with J. multifida in earlier based on multilocus marker analysis, was clustered with J. curcas in the present analysis. The sequence data generated in the present investigation will help for further studies in intraspecies population, and their phylogenetic analysis, biogeographical, molecular evolution studies and also pave way for future phylogenetic and/or evolution studies among the other groups belongs to the family Euphorbiaceae.
麻风树属属于大戟科，具有重要的经济意义。本研究旨在利用核糖体DNA ITS序列（nrDNA ITS）了解广泛分布于印度的七个物种（麻疯树、腺叶麻风树、棉叶麻风树、全缘叶麻风树、多裂叶麻风树、珊瑚麻风树和坦焦尔麻风树）之间的系统发育关系，并将结果与先前报道的同一属的多基因座标记分析系统进行比较。测序得到的nrDNA ITS区域大小变化范围较窄，为647至654 bp。麻风树属的总体平均遗传距离（GD）为0.385。腺叶麻风树和多裂叶麻风树之间的种间遗传距离最高（0.419）。棉叶麻风树和坦焦尔麻风树之间的种间遗传距离最小（0.085）。珊瑚麻风树的种内遗传距离最高（0.011），棉叶麻风树的种内遗传距离最小（0.002）。使用nrDNA ITS序列获得的系统发育树与先前报道的使用多基因座标记系统获得的系统发育树显示出一致性，只是有一些小的差异。本研究还强烈支持麻疯树和全缘叶麻风树在系统发育上的高度亲缘关系。唯一的例外是珊瑚麻风树，在早期基于多基因座标记分析它与多裂叶麻风树聚类，而在本分析中它与麻疯树聚类。本研究中产生的序列数据将有助于进一步开展种内群体、系统发育分析、生物地理学、分子进化研究，也为大戟科其他类群未来的系统发育和/或进化研究铺平道路。
