Sudheer Pamidiamarri D V N, Pandya Nirali, Reddy Muppala P, Radhakrishnan T
Discipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute, Bhavnagar, Gujarat, India.
Mol Biol Rep. 2009 May;36(5):901-7. doi: 10.1007/s11033-008-9261-0. Epub 2008 Apr 30.
Genus Jatropha with 172 species having significant economic importance belongs to the family Euphorbiaceae. There are no reports on molecular characterization and phylogenetic relationship among the species of Jatropha. Hence, the present study was undertaken to assess the extent of genetic variability that exist and also to establish phylogenetic relationship among Jatropha curcas, J. glandulifera, J. gossypifolia, J. integerrima, J. multifida, J. podagrica and J. tanjorensis using RAPD and AFLP. The percentage of loci that are polymorphic among the species studied was found to be 97.74% by RAPD and 97.25% by AFLP. The mean percentage of polymorphism (PP) was found to be 68.48 by RAPD and 71.33 by AFLP. The phylogram generated with RAPD and AFLP data showed maximum similarity. With the generated data maximum relatedness was found between J. curcas and J. integerrima this may be the reason for the success of inter hybrid crosses between these two species. Neither RAPD nor AFLP data generated in this study supports the view of J. tanjorensis, a natural interspecific hybrid between J. curcas and J. gossypifolia. The present study concludes that both RAPD and AFLP techniques are comparable in divergence studies of Jatropha species. The markers generated by RAPD and AFLP can be employed efficiently for interspecific hybrids identification, marker assisted selection and genetic resource management.
麻风树属属于大戟科，有172个具有重要经济价值的物种。目前尚无关于麻风树属物种间分子特征和系统发育关系的报道。因此，本研究旨在评估现有遗传变异的程度，并利用随机扩增多态性DNA（RAPD）和扩增片段长度多态性（AFLP）技术，确定麻疯树、腺叶麻风树、棉叶麻风树、全缘叶麻风树、多裂叶麻风树、佛肚树和坦焦尔麻风树之间的系统发育关系。通过RAPD分析，所研究物种中多态性位点的百分比为97.74%，通过AFLP分析为97.25%。RAPD分析的平均多态性百分比（PP）为68.48，AFLP分析为71.33。由RAPD和AFLP数据生成的系统发育树显示出最大的相似性。根据生成的数据，发现麻疯树和全缘叶麻风树之间的亲缘关系最大，这可能是这两个物种间杂交成功的原因。本研究中生成的RAPD和AFLP数据均不支持坦焦尔麻风树是麻疯树和棉叶麻风树之间自然种间杂种的观点。本研究得出结论，在麻风树属物种的分歧研究中，RAPD和AFLP技术具有可比性。RAPD和AFLP产生的标记可有效地用于种间杂种鉴定、标记辅助选择和遗传资源管理。
