Liu Longzhou, Cai Run, Yuan Xiaojun, He Huanle, Pan Junsong
School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200240, China.
Sci China C Life Sci. 2008 Nov;51(11):1003-8. doi: 10.1007/s11427-008-0110-0. Epub 2008 Nov 7.
The cucumber lines, S94 (Northern China open-field type, powdery mildew (PM) susceptible) and S06 (European greenhouse type, PM resistant), and their F(6:7) populations were used to investigate PM resistance under seedling spray inoculation in 2005/Autumn and 2006/Spring. QTL analysis was undertaken based on a constructed molecular linkage map of the corresponding F(6) population using composite interval mapping. A total of four QTLs (pm1.1, pm2.1, pm4.1 and pm6.1) for PM resistance were identified and located on LG 1, 2, 4 and 6, respectively, explaining 5.2%-21.0% of the phenotypic variation. Three consistent QTLs (pm1.1, pm2.1 and pm4.1) were detected under the two test conditions. The QTL pm6.1 was only identified in 2005/Autumn. The total phenotypic variation explained by the QTLs was 52.0% and 42.0% in 2005/Autumn and 2006/Spring, respectively. Anchor markers tightly linked to those loci (<5 cM) could lay a basis for both molecular marker-assisted breeding and map-based gene cloning of the PM-resistance gene in cucumber.
黄瓜品系S94（华北露地型，感白粉病（PM））和S06（欧洲温室型，抗PM）及其F(6:7)群体于2005年秋季和2006年春季用于研究幼苗喷雾接种条件下的抗白粉病性。基于构建的相应F(6)群体的分子连锁图谱，采用复合区间作图法进行QTL分析。共鉴定出4个抗白粉病的QTL（pm1.1、pm2.1、pm4.1和pm6.1），分别位于LG 1、2、4和6上，解释了5.2%-21.0%的表型变异。在两种测试条件下检测到3个一致的QTL（pm1.1、pm2.1和pm4.1）。QTL pm6.1仅在2005年秋季被鉴定出。这些QTL在2005年秋季和2006年春季分别解释了52.0%和42.0%的总表型变异。与这些位点紧密连锁（<5 cM）的锚定标记可为黄瓜抗白粉病基因的分子标记辅助育种和基于图谱的基因克隆奠定基础。
