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抗与感白粉病栽培黄瓜（Cucumis sativus L.）品种的比较分析，揭示其对瓜类白粉病菌侵染的代谢响应。

Comparative analysis of powdery mildew resistant and susceptible cultivated cucumber (Cucumis sativus L.) varieties to reveal the metabolic responses to Sphaerotheca fuliginea infection.

机构信息

Institute of Vegetable, Zhejiang Academy of Agriculture Sciences, Hangzhou, China.

出版信息

BMC Plant Biol. 2021 Jan 7;21(1):24. doi: 10.1186/s12870-020-02797-3.

DOI:10.1186/s12870-020-02797-3
PMID:33413112
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7791650/
Abstract

BACKGROUND

Cucumber (Cucumis sativus L.) is a widely planted vegetable crop that suffers from various pathogen infections. Powdery mildew (PM) is typical disease caused by Sphaerotheca fuliginea infection and destroys the production of cucumber. However, the metabolic responses to S. fuliginea infection are largely unknown.

RESULTS

In our study, a PM resistant variety 'BK2' and a susceptible variety 'H136' were used to screen differentially accumulated metabolites (DAMs) and differentially expressed genes (DEGs) under S. fuliginea infection. Most of DEGs and DAMs were enriched in several primary and secondary metabolic pathways, including flavonoid, hormone, fatty acid and diterpenoid metabolisms. Our data showed that many flavonoid-related metabolites were significantly accumulated in BK2 rather than H136, suggesting an essential role of flavonoids in formation of resistant quality. Changes in expression of CYP73A, CYP81E1, CHS, F3H, HCT and F3'M genes provided a probable explanation for the differential accumulation of flavonoid-related metabolites. Interestingly, more hormone-related DEGs were detected in BK2 compared to H136, suggesting a violent response of hormone signaling pathways in the PM-resistant variety. The number of fatty acid metabolism-related DAMs in H136 was larger than that in BK2, indicating an active fatty acid metabolism in the PM-susceptible variety.

CONCLUSIONS

Many differentially expressed transcription factor genes were identified under S. fuliginea infection, providing some potential regulators for the improvement of PM resistance. PM resistance of cucumber was controlled by a complex network consisting of various hormonal and metabolic pathways.

摘要

背景

黄瓜（Cucumis sativus L.）是一种广泛种植的蔬菜作物，易受多种病原菌感染。白粉病（PM）是由 Sphaerotheca fuliginea 感染引起的典型病害，会破坏黄瓜的产量。然而，其对 S. fuliginea 感染的代谢反应在很大程度上尚不清楚。

结果

在本研究中，使用抗白粉病品种‘BK2’和感病品种‘H136’筛选受 S. fuliginea 感染后差异积累代谢物（DAMs）和差异表达基因（DEGs）。大多数 DEGs 和 DAMs 富集在几个初级和次级代谢途径中，包括类黄酮、激素、脂肪酸和二萜代谢途径。我们的数据表明，许多类黄酮相关代谢物在 BK2 中明显积累，而在 H136 中则不然，这表明类黄酮在抗性品质形成中具有重要作用。CYP73A、CYP81E1、CHS、F3H、HCT 和 F3'M 基因表达的变化为类黄酮相关代谢物的差异积累提供了一个可能的解释。有趣的是，与 H136 相比，BK2 中检测到更多与激素相关的 DEGs，这表明在抗白粉病品种中激素信号通路发生了剧烈反应。H136 中与脂肪酸代谢相关的 DAMs 数量多于 BK2，这表明在感病品种中脂肪酸代谢活跃。

结论

在 S. fuliginea 感染下鉴定出许多差异表达的转录因子基因，为提高白粉病抗性提供了一些潜在的调控因子。黄瓜对白粉病的抗性由一个由各种激素和代谢途径组成的复杂网络控制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e96/7791650/fc7de9d62aa2/12870_2020_2797_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e96/7791650/864cd7b6fa43/12870_2020_2797_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e96/7791650/01e89d1d2f05/12870_2020_2797_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e96/7791650/f35479992a70/12870_2020_2797_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e96/7791650/fc7de9d62aa2/12870_2020_2797_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e96/7791650/864cd7b6fa43/12870_2020_2797_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e96/7791650/3dfe194bf717/12870_2020_2797_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e96/7791650/a6bff26b3e04/12870_2020_2797_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e96/7791650/16278982671a/12870_2020_2797_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e96/7791650/01e89d1d2f05/12870_2020_2797_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e96/7791650/f35479992a70/12870_2020_2797_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e96/7791650/fc7de9d62aa2/12870_2020_2797_Fig7_HTML.jpg

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