Haferkamp Axel, Bedke Jens, Vetter Caroline, Pritsch Maria, Wagener Nina, Buse Stephan, Crnkovic-Mertens Irena, Hoppe-Seyler Karin, Macher-Goeppinger Stephan, Hoppe-Seyler Felix, Autschbach Frank, Hohenfellner Markus
Department of Urology, University of Heidelberg, Heidelberg, Germany.
BJU Int. 2008 Dec;102(11):1700-6. doi: 10.1111/j.1464-410X.2008.07910.x. Epub 2008 Sep 18.
To assess the protein expression of Livin, an apoptosis inhibitor, in renal cell carcinoma (RCC) and to determine its prognostic relevance.
Immunohistochemical staining for Livin was performed in tissue microarrays (TMAs), including tumour tissue cores, from patients with RCC who had undergone renal surgery. In 682 TMAs cytoplasmatic staining intensity and nuclear staining quantity were evaluated, and the association of Livin expression with progression-free survival (PFS) and cancer-specific survival (CSS) was analysed with a multivariate Cox regression model.
Over a median (range) follow-up of 5.2 (0-16.1) years, 204 patients (28%) had died from their disease. The CSS rates at 1 and 5 years for the entire cohort was 88% and 71%. Cytoplasmatic Livin staining was absent in 516 (76%) specimens; staining was positive in 166 (24%) specimens. Weak nuclear Livin staining (<or=25%) was present in 571 (84%) specimens, strong nuclear staining (26-100%) in 111 (16%). In multivariate analysis, high (>25%) nuclear Livin expression was a favourable independent predictor of PFS and CSS even after adjusting for tumour stage, Fuhrman grade, age, sex and Karnofsky severity rating. Cytoplasmatic Livin expression did not offer additional prognostic information.
High nuclear Livin expression is a favourable independent predictor of PFS and CSS in patients with RCC.
评估凋亡抑制因子Livin在肾细胞癌（RCC）中的蛋白表达，并确定其预后相关性。
对接受肾脏手术的RCC患者的组织微阵列（TMA）进行Livin免疫组化染色，TMA包括肿瘤组织核心。在682个TMA中评估细胞质染色强度和细胞核染色数量，并使用多变量Cox回归模型分析Livin表达与无进展生存期（PFS）和癌症特异性生存期（CSS）的相关性。
在中位（范围）5.2（0 - 16.1）年的随访期内，204例患者（28%）死于疾病。整个队列1年和5年的CSS率分别为88%和71%。516个（76%）标本中无细胞质Livin染色；166个（24%）标本染色呈阳性。571个（84%）标本有弱细胞核Livin染色（≤25%），111个（16%）有强细胞核染色（26 - 100%）。在多变量分析中，即使在调整肿瘤分期、Fuhrman分级、年龄、性别和卡诺夫斯基严重程度评分后，高（>25%）细胞核Livin表达仍是PFS和CSS的有利独立预测因素。细胞质Livin表达未提供额外的预后信息。
高细胞核Livin表达是RCC患者PFS和CSS的有利独立预测因素。
