Harada M, Nishitani H, Shirahama T, Koga K, Miura I
Department of Radiology, School of Medicine, Tokushima University, Japan.
Gan To Kagaku Ryoho. 1991 Jan;18(1):75-80.
5-Fluorouracil (5-FU) metabolism on tumors was studied by in-vivo 19F-MRS (magnetic resonance spectroscopy). In this study, two kinds of tumors were used, i.e., Yoshida sarcoma implanted subcutaneously to the abdomen of rats and drug-induced tumors in the rats livers. Sequential 19F spectra were obtained just after 150 mg/kg 5-FU injected intravenously. In Yoshida sarcoma, the accumulation of 5-FU was observed and disappearance of 5-FU was slower compared to the normal tissue. However, synthesis of fluoronucleotides (Fnct) could not be detected. In drug-induced liver tumors, the peak of fluoro-beta-alanine (FBAL) was observed. Disappearance of 5-FU and catabolism to FBAL in the liver tumors group were slower compared to the normal liver. Synthesis of Fnct did not increase in the liver tumor group. The results in the liver tumor group are considered to be the confined result of the hepatocytes and tumors cells. It was considered that the delayed catabolism to FBAL in the liver tumor group showed metabolic dysfunction of the liver. Also the synthesis of Fnct in tumors could not be detected by in-vivo 19F-MRS. 19F-MRS method could not detect Fnct in tumors in-vivo. However, the accumulation of 5-FU could be assessed by this method. It is expected that the evaluation of 5-FU pooling in tumors could be used for the index of chemotherapeutic effect.
通过体内¹⁹F磁共振波谱（MRS）研究了5-氟尿嘧啶（5-FU）在肿瘤中的代谢情况。在本研究中，使用了两种肿瘤，即皮下植入大鼠腹部的吉田肉瘤和大鼠肝脏中的药物诱导肿瘤。静脉注射150mg/kg 5-FU后立即获得连续的¹⁹F谱。在吉田肉瘤中，观察到5-FU的蓄积，且与正常组织相比，5-FU的消失较慢。然而，未检测到氟核苷酸（Fnct）的合成。在药物诱导的肝肿瘤中，观察到氟-β-丙氨酸（FBAL）的峰。与正常肝脏相比，肝肿瘤组中5-FU的消失和向FBAL的分解代谢较慢。肝肿瘤组中Fnct的合成没有增加。肝肿瘤组的结果被认为是肝细胞和肿瘤细胞的局限性结果。据认为，肝肿瘤组中向FBAL的分解代谢延迟表明肝脏存在代谢功能障碍。此外，体内¹⁹F-MRS无法检测到肿瘤中Fnct的合成。¹⁹F-MRS方法无法在体内检测到肿瘤中的Fnct。然而，通过该方法可以评估5-FU在肿瘤中的蓄积情况。预计评估肿瘤中5-FU的蓄积可作为化疗效果的指标。
