Otsuka H, Harada M, Koga K, Nishitani H
Department of Radiology, Tokushima University, Japan.
Magn Reson Imaging. 1999 Feb;17(2):283-90. doi: 10.1016/s0730-725x(98)00077-0.
The purpose of this study was to observe the effects of hepatic impairment on the metabolism of fructose and 5-fluorouracil (5-FU) in fatty liver models using in vivo 31P-MRS and 19F-MRS and to compare the results. In addition, we compared the results to those of other conventional tests such as laboratory examinations, imaging and pathology. Male SIc:Wistar rats were examined on BEM170/200 (4.7 T, Otsuka Electronics, USA) with 17-mm diameter surface coil. Fatty liver was induced by a choline deficient diet (CD diet) for 2 weeks. 31P-MRS were obtained for 90 min after intravenous (i.v.) injection of 1 g/kg of fructose and 19F-MRS were measured for 100 min after i.v. injection of 100 mg/kg of 5-FU. 1H-MRS and 1H-MRI were also performed. On 31P-MRS, there was no statistical difference in the time course of phosphomonoester (PME), adenosine triphosphate (ATP), and inorganic phosphate (Pi) between CD diet group and control group. On 19F-MRS, we detected high peak of fluoronucleotide (Fnct) and suppressed peak of alpha-fluoro-beta-alanine (FBAL) in CD diet group. We showed the metabolism of fructose and 5-FU by 31P-MRS and 19F-MRS, respectively. There was no difference in fructose metabolism but we observed increased fluoronucleotide and decreased a-fluoro-b-alanine in 5-FU metabolism of fatty liver. We speculate that the effects of hepatic impairment in fatty liver may be more severe on 5-FU metabolism and the increased fluoronucleotide may reflect cell proliferation.
本研究的目的是使用体内31P磁共振波谱（MRS）和19F MRS观察肝损伤对脂肪肝模型中果糖和5-氟尿嘧啶（5-FU）代谢的影响，并比较结果。此外，我们将结果与其他传统检测方法（如实验室检查、影像学和病理学）的结果进行了比较。雄性SIc:Wistar大鼠在配备17毫米直径表面线圈的BEM170/200（4.7T，大冢电子，美国）上进行检查。通过胆碱缺乏饮食（CD饮食）诱导脂肪肝2周。静脉注射1g/kg果糖后90分钟获取31P MRS，静脉注射100mg/kg 5-FU后100分钟测量19F MRS。还进行了1H MRS和1H磁共振成像（MRI）。在31P MRS上，CD饮食组和对照组之间的磷酸单酯（PME）、三磷酸腺苷（ATP）和无机磷酸盐（Pi）的时间进程没有统计学差异。在19F MRS上，我们在CD饮食组中检测到氟核苷酸（Fnct）的高峰和α-氟-β-丙氨酸（FBAL）的抑制峰。我们分别通过31P MRS和19F MRS展示了果糖和5-FU的代谢。果糖代谢没有差异，但我们观察到脂肪肝的5-FU代谢中氟核苷酸增加，α-氟-β-丙氨酸减少。我们推测肝损伤在脂肪肝中对5-FU代谢的影响可能更严重，并且氟核苷酸的增加可能反映细胞增殖。
