Xin Hai-liang, Hou Yin-huan, Li Min, Lu Jin-cai, Ling Chang-quan
Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Zhong Xi Yi Jie He Xue Bao. 2008 Nov;6(11):1174-7. doi: 10.3736/jcim20091113.
To determine alpha-linolenic acid and linoleic acid in extract of Portulaca oleracea L. by high performance liquid chromatography (HPLC).
The determination was done with a Shim-pack CLC-ODS (250 mm x 4.6 mm, 5 microm) and a DIKMA Easyguard C18 (10 mm x 4.6 mm). Elution was employed with the mobile phase of methanol-acetonitrile-0.5% phosphonic acid (60:22:18) at flow rate of 1.1 ml/min. Column temperature was 26 degrees centigrade. Detection wavelength was 210 nm. Injection volume was 25 microl.
The standard curves of alpha-linolenic acid and linoleic acid were linear in the range 0.016 2 to 0.194 4 mg/ml and 0.016 9 to 0.203 0 mg/ml, respectively. The regression equations were A=2.915 8 x 10(7) C+12,250.9, r=0.999 9 and A=1.366 4 x 10(7) C-9,759.39, r=0.999 9, respectively. The average recovery rates were 100.5% and 100.8%, respectively.
The present method (HPLC) may be considered to be reliable and simple for the determination of alpha-linolenic acid and linoleic acid in extract of Portulaca oleracea L.
采用高效液相色谱法（HPLC）测定马齿苋提取物中的α-亚麻酸和亚油酸。
使用Shim-pack CLC-ODS（250 mm×4.6 mm，5微米）和DIKMA Easyguard C18（10 mm×4.6 mm）色谱柱。以甲醇-乙腈-0.5%膦酸（60:22:18）为流动相进行洗脱，流速为1.1 ml/min。柱温为26℃。检测波长为210 nm。进样量为25微升。
α-亚麻酸和亚油酸的标准曲线在0.016 2至0.194 4 mg/ml和0.016 9至0.203 0 mg/ml范围内分别呈线性。回归方程分别为A = 2.915 8×10⁷C + 12,250.9，r = 0.999 9和A = 1.366 4×10⁷C - 9,759.39，r = 0.999 9。平均回收率分别为100.5%和100.8%。
本方法（HPLC）可认为是可靠且简便的，用于测定马齿苋提取物中的α-亚麻酸和亚油酸。
