Chen Juan, Shi Yan-Ping, Liu Jing-Yan
Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Tianshui Road 342, Lanzhou 730000, China.
J Chromatogr A. 2003 Jun 27;1003(1-2):127-32. doi: 10.1016/s0021-9673(03)00786-6.
A simple, rapid and accurate high-performance liquid chromatographic (HPLC) technique coupled with photodiode array (PDA) detection was developed for the simultaneous determination of noradrenaline (NA) and dopamine (DA) in Chinese herbal plant extracts from the different parts of Portulaca oleracea L. The effects of various parameters, such as sodium dodecyl sulfate (SDS) concentration, pH value and proportion of methanol on chromatographic behavior of the analytes (NA and DA) were investigated. Separation of NA and DA was achieved within 10 min by a mobile phase consisting of 70% (v/v) methanol and 0.02 M potassium dihydrogen phosphate solution, which contained 30 mM SDS and was adjusted to pH 3.0 with H3PO4. NA and DA showed good linear relationships in the range of 0.004-6.00 microg and 0.011-8.25 microg respectively. The correlation coefficients of the calibration curve for the analytes exceeded 0.999. The detection limits for NA and DA were 0.40 ng and 0.55 ng, at a signal-to-noise ratio of 3, respectively. Moreover, the optimized HPLC method was employed to analyze three different parts of Portulaca oleracea L.
建立了一种简单、快速且准确的高效液相色谱（HPLC）技术，结合光电二极管阵列（PDA）检测，用于同时测定马齿苋不同部位中草药提取物中的去甲肾上腺素（NA）和多巴胺（DA）。研究了各种参数，如十二烷基硫酸钠（SDS）浓度、pH值和甲醇比例对分析物（NA和DA）色谱行为的影响。通过由70%（v/v）甲醇和0.02 M磷酸二氢钾溶液组成的流动相，在10分钟内实现了NA和DA的分离，该流动相含有30 mM SDS，并用H3PO4调节至pH 3.0。NA和DA分别在0.004 - 6.00 μg和0.011 - 8.25 μg范围内呈现良好的线性关系。分析物校准曲线的相关系数超过0.999。NA和DA在信噪比为3时的检测限分别为0.40 ng和0.55 ng。此外，采用优化后的HPLC方法对马齿苋的三个不同部位进行了分析。
