Nielsen B, Jessen P, Hokland P
University Department of Medicine and Haematology, Aarhus Amtssygehus, Denmark.
Eur J Haematol. 1991 Jan;46(1):26-32. doi: 10.1111/j.1600-0609.1991.tb00509.x.
In order to characterize the effect of T lymphocytes on the in vitro growth of erythroid progenitor cells (EPC), we have optimized an assay for evaluating the influence of autologous T-lymphocyte clones on BFU-e, grown from PBMNCs depleted of T lymphocytes. Testing a large number of T-lymphocyte clones from 2 individuals we found that, irrespective of immunological phenotype, all clones tested were found to enhance the growth of BFU-e. Thus, no evidence for a functional dichotomy between CD4+ and CD8+ T lymphocytes was found in this system. The enhancing effect worked across a histocompatibility barrier and was, at least in part, mediated by soluble factors. We therefore conclude that the role of the majority of cloned normal T lymphocytes in the regulation of the in vitro growth of EPC is a supportive one.
为了描述T淋巴细胞对红系祖细胞（EPC）体外生长的影响，我们优化了一种检测方法，用于评估自体T淋巴细胞克隆对从去除T淋巴细胞的外周血单个核细胞（PBMNCs）中培养的爆式红系集落形成单位（BFU-e）的影响。通过检测来自2名个体的大量T淋巴细胞克隆，我们发现，无论免疫表型如何，所有测试的克隆均能增强BFU-e的生长。因此，在该系统中未发现CD4 +和CD8 + T淋巴细胞之间存在功能二分法的证据。这种增强作用跨越了组织相容性屏障，并且至少部分是由可溶性因子介导的。因此，我们得出结论，大多数克隆的正常T淋巴细胞在EPC体外生长调节中的作用是支持性的。
