Karkadeh（洛神花）种子的近程组成和种子蛋白分离物的一些功能特性受 pH 值和 NaCl 的影响。

Proximate composition of Karkadeh (Hibiscus sabdariffa) seeds and some functional properties of seed protein isolate as influenced by pH and NaCl.

机构信息

University of Botswana, Gaborone, Botswana.

出版信息

Int J Food Sci Nutr. 2009 May;60(3):183-94. doi: 10.1080/09637480701664399. Epub 2008 Nov 6.

Abstract

Seeds of an inbred line (B-11-90) of Karkadeh (Hibiscus sabdariffa) were investigated for their proximate composition (AOAC methods), nitrogen solubility and protein isolate (Karkadeh seed protein isolates [KSPI]) functional properties (standard methods). The fat and protein contents of the seeds were 22.43% and 32.46%, respectively. Nitrogen solubility was good in both water and 1.0 M NaCl at alkaline pH rather than at acidic pH, with better solubility at higher pH levels in water than in 1.0 M NaCl. The functional properties of the KSPI were as follows: water absorption capacity, 181 ml/100 g; fat absorption capacity, 110 ml/100 g; bulk density, 0.77 g/ml; and apparent viscosity (at 20 degrees C), 13.42 cps. KSPI showed a maximum foaming capacity at pH 12 and 1.6 M NaCl, a maximum emulsification capacity at pH 11 and 1.8 M NaCl, and a weaker foam stability at neutral pH than at acidic or alkaline pH, with a better foam stability at alkaline pH. The foam stability was considerably improved by treatment with 1.6 M NaCl.

摘要

对 Karkadeh（洛神花）的一个自交系（B-11-90）种子的近等基因系进行了研究，内容包括其近似成分（AOAC 方法）、氮溶解度和蛋白质分离物（Karkadeh 种子蛋白分离物[KSPI]）的功能特性（标准方法）。种子的脂肪和蛋白质含量分别为 22.43%和 32.46%。氮在碱性 pH 条件下的水中和 1.0 M NaCl 中的溶解度都很好，而在酸性 pH 条件下的溶解度则较差，在水中的溶解度在较高 pH 条件下比在 1.0 M NaCl 中更好。KSPI 的功能特性如下：水吸收能力为 181 ml/100 g；脂肪吸收能力为 110 ml/100 g；堆积密度为 0.77 g/ml；表观粘度（在 20°C 下）为 13.42 cps。KSPI 在 pH 12 和 1.6 M NaCl 下表现出最大的起泡能力，在 pH 11 和 1.8 M NaCl 下表现出最大的乳化能力，在中性 pH 下的泡沫稳定性比在酸性或碱性 pH 下的泡沫稳定性更差，在碱性 pH 下的泡沫稳定性更好。用 1.6 M NaCl 处理可以显著提高泡沫稳定性。