Deleuze S, Dubois C S, Caillaud M, Bruneau B, Goudet G, Duchamp G
Faculté de Médecine Vétérinaire, Département des Sciences Cliniques-Clinique Equine, Université de Liège, Liège, Belgium.
Reprod Domest Anim. 2010 Feb;45(1):1-7. doi: 10.1111/j.1439-0531.2008.01122.x. Epub 2008 Oct 28.
Contents The effect of cysteamine on in vitro nuclear and cytoplasmic maturation of equine oocytes collected by transvaginal ultrasound guided follicular aspiration was assessed. Oocytes were matured in vitro with (cysteamine group) or without (control group) cysteamine. The nuclear stage after DNA Hoechst staining, penetration rates after two different in vitro fertilization (IVF) techniques (IVF media with ionophore and Hepes buffer with heparin) and the embryo yield following oocyte intra-oviductal transfer were used as a criterion for assessing nuclear and cytoplasmic maturation, respectively. Contrary to the data described in other domestic species, there was no effect of cysteamine on in vitro nuclear maturation, IVF or in vivo embryonic development under our conditions. Ovum pick up yields (52%) and maturation rates (control group: 47% and cysteamine group: 55%) were similar to those previously reported. From 57 oocytes transferred to the oviduct in each group, the number of embryos collected was 10 (17%) in the control group and five in the cysteamine group (9%). Those two percentages were not statistically different (p > 0.05). No effect of IVF technique was seen on the success rate (6%) in each group.
内容 评估了半胱胺对经阴道超声引导卵泡抽吸采集的马卵母细胞体外核成熟和胞质成熟的影响。卵母细胞在有（半胱胺组）或无（对照组）半胱胺的情况下进行体外成熟。DNA Hoechst染色后的核阶段、两种不同体外受精（IVF）技术（含离子载体的IVF培养基和含肝素的Hepes缓冲液）后的穿透率以及卵母细胞输卵管内移植后的胚胎产量分别用作评估核成熟和胞质成熟的标准。与其他家畜物种中描述的数据相反，在我们的条件下，半胱胺对体外核成熟、IVF或体内胚胎发育没有影响。采卵产量（52%）和成熟率（对照组：47%，半胱胺组：55%）与先前报道的相似。每组向输卵管移植57个卵母细胞，对照组收集到的胚胎数量为10个（17%），半胱胺组为5个（9%）。这两个百分比无统计学差异（p>0.05）。未观察到IVF技术对每组成功率（6%）有影响。
