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Zygote. 2007 May;15(2):93-102. doi: 10.1017/S0967199406004035.
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Cysteamine supplementation during in vitro maturation and embryo culture: a useful tool for increasing the efficiency of bovine in vitro embryo production.体外成熟和胚胎培养过程中补充半胱胺：提高牛体外胚胎生产效率的有用工具。
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引用本文的文献

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1
In vitro maturation: a five-year experience.体外成熟：五年经验。
Acta Obstet Gynecol Scand. 2012 Jan;91(1):22-27. doi: 10.1111/j.1600-0412.2011.01299.x. Epub 2011 Nov 9.
2
Assessment of oxidative stress-induced DNA damage by immunoflourescent analysis of 8-oxodG.通过对8-氧代脱氧鸟苷进行免疫荧光分析评估氧化应激诱导的DNA损伤。
Methods Cell Biol. 2011;103:99-113. doi: 10.1016/B978-0-12-385493-3.00005-X.
3
Regulation of reactive oxygen species in stem cells and cancer stem cells.干细胞和肿瘤干细胞中活性氧的调节。
J Cell Physiol. 2012 Feb;227(2):421-30. doi: 10.1002/jcp.22764.
4
The promise of in vitro maturation in assisted reproduction and fertility preservation.体外成熟在辅助生殖和生育力保存中的应用前景。
Semin Reprod Med. 2011 Jan;29(1):24-37. doi: 10.1055/s-0030-1268701. Epub 2011 Jan 4.
5
The roles of cellular reactive oxygen species, oxidative stress and antioxidants in pregnancy outcomes.细胞活性氧、氧化应激和抗氧化剂在妊娠结局中的作用。
Int J Biochem Cell Biol. 2010 Oct;42(10):1634-50. doi: 10.1016/j.biocel.2010.06.001. Epub 2010 Jun 19.
6
Oocyte glutathione and fertilisation outcome of Macaca nemestrina and Macaca fascicularis in in vivo- and in vitro-matured oocytes.猕猴体内和体外成熟卵母细胞的谷胱甘肽与受精结果，针对豚尾猕猴和食蟹猕猴
Reprod Fertil Dev. 2010;22(6):1032-40. doi: 10.1071/RD09308.
7
Laboratory and embryological aspects of hCG-primed in vitro maturation cycles for patients with polycystic ovaries.多囊卵巢患者人绒毛膜促性腺激素（hCG）触发的体外成熟周期的实验室和胚胎学方面。
Hum Reprod Update. 2010 Nov-Dec;16(6):675-89. doi: 10.1093/humupd/dmq014. Epub 2010 May 26.
8
The roles of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38 MAPK) in aged pig oocytes.
J Reprod Dev. 2009 Feb;55(1):75-82. doi: 10.1262/jrd.20061. Epub 2008 Nov 20.
9
Influence of cysteamine on in vitro maturation, in vitro and in vivo fertilization of equine oocytes.半胱胺对马属动物卵母细胞体外成熟、体外及体内受精的影响。
Reprod Domest Anim. 2010 Feb;45(1):1-7. doi: 10.1111/j.1439-0531.2008.01122.x. Epub 2008 Oct 28.
10
Glutathione and apoptosis.谷胱甘肽与细胞凋亡
Free Radic Res. 2008 Aug;42(8):689-706. doi: 10.1080/10715760802317663.

在兔卵母细胞体外成熟（IVM）过程中补充半胱胺可提高胞浆内精子注射后的发育能力。

Cysteamine supplementation during in vitro maturation (IVM) of rabbit oocyte improves the developmental capacity after intracytoplasmic sperm injection.

作者信息

Izumi Hiroki, Miyamoto Yuki, Mori Tatsufumi, Hashigami Yuka, Chiba Yasutaka, Teramura Takeshi, Hashimoto Shu, Fukuda Kanji, Morimoto Yoshiharu, Hosoi Yoshihiko

机构信息

Department of Biology Oriented Science and Technology Kinki University Wakayama Japan.

IVF Namba Clinic Osaka Japan.

出版信息

Reprod Med Biol. 2013 Jun 21;12(4):179-185. doi: 10.1007/s12522-013-0157-x. eCollection 2013 Oct.

DOI:10.1007/s12522-013-0157-x
PMID:29699144
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5907106/
Abstract

PURPOSE

Current approaches to in vitro maturation (IVM) may result in low efficiency and inadequate quality of the oocytes due to insufficient cytoplasmic maturation. Although positive effects of the cysteamine supplementation in IVM medium for oocyte nuclear maturation or male pronuclear formation have been confirmed, it is still controversial whether the cysteamine addition affects embryo development after IVM. We aimed here to confirm the effect of cysteamine addition into IVM medium for subsequent embryo development in vitro.

METHODS

We administered the cysteamine to the IVM culture of rabbit immature oocytes at various concentrations and observed the developmental rate, speed to reach blastocyst stage and cell numbers at the blastocyst stage.

RESULTS

Cysteamine supplementation improved developmental rate to blastocyst stage of the IVM oocytes. On the other hand, addition of glutathione (GSH) inhibitor buthionine sulfoximine inhibited GSH accumulation in the oocytes and subsequent embryo development to the blastocyst stage.

CONCLUSIONS

Controlling the GSH quantity of IVM oocytes may be an important factor for success of embryo development, and it is quite probable that a cysteamine supplementation can contribute to an increase of GSH content in oocyte.

摘要

目的

由于细胞质成熟不足，目前的体外成熟（IVM）方法可能导致卵母细胞效率低下和质量欠佳。尽管已证实向IVM培养基中添加半胱胺对卵母细胞核成熟或雄原核形成具有积极作用，但添加半胱胺是否会影响IVM后的胚胎发育仍存在争议。我们的目的是在此确认向IVM培养基中添加半胱胺对后续体外胚胎发育的影响。

方法

我们以不同浓度将半胱胺应用于兔未成熟卵母细胞的IVM培养，并观察发育率、达到囊胚阶段的速度以及囊胚阶段的细胞数量。

结果

添加半胱胺提高了IVM卵母细胞发育至囊胚阶段的比率。另一方面，添加谷胱甘肽（GSH）抑制剂丁硫氨酸亚砜胺可抑制卵母细胞中GSH的积累以及随后胚胎发育至囊胚阶段。

结论

控制IVM卵母细胞的GSH量可能是胚胎发育成功的一个重要因素，并且补充半胱胺很可能有助于增加卵母细胞中的GSH含量。