Salvetti N R, Baravalle C, Mira G A, Gimeno E J, Dallard B E, Rey F, Ortega H H
Department of Anatomy and Histology, Center for Experimental Biology and Laboratory Animals Sciences, Faculty of Veterinary Sciences, National University of Litoral, Argentina.
Reprod Domest Anim. 2009 Oct;44(5):805-14. doi: 10.1111/j.1439-0531.2008.01086.x. Epub 2008 Oct 22.
The purpose of this study was to estimate the expression and relative amounts of estrogen (ER) and progesterone receptors (PR) and their isoforms as well as heat shock protein 70 (HSP70) in ovaries of rats with induced cystic ovarian disease (COD). Primary, secondary, tertiary, atretic and cystic follicles were evaluated by immunohistochemistry and total ovarian proteins were analyzed by Western blot. In the granulosa layer, growing and cystic follicles in the treated group have a higher expression of ERalpha than growing follicles of control individuals. In the theca interna layer, tertiary follicles presented a significantly higher expression of ERalpha in the treated group. An increase in total ERalpha protein was detected in the treated group. Granulosa cells of all growing, atretic and cystic follicles show a lower expression of ERbeta in animals with COD, and the total protein expression of ERbeta was lower in this group. The expression of PR was lower in the granulosa cell layer of tertiary and cystic follicles in treated animals, and theca interna layer had less intense immunostaining in this group. Although there were no differences in the expression of PR-B by Western blotting, the expression of PR-A was higher and the expression of PR-C was smaller in the treated group. An intense HSP70 immunostaining was observed in the cells of cystic follicles. By Western blotting, higher protein expression of HSP70 was detected in the ovarian samples of the control group than those of the treated ones. Ovaries of animals with COD exhibited an altered steroid receptor expression and subtype balance as compared with control animals, and an increase in HSP70 immunoexpression.
本研究的目的是评估诱导性多囊卵巢疾病（COD）大鼠卵巢中雌激素（ER）和孕激素受体（PR）及其亚型以及热休克蛋白70（HSP70）的表达和相对含量。通过免疫组织化学评估初级、次级、三级、闭锁和囊性卵泡，并通过蛋白质印迹法分析卵巢总蛋白。在颗粒层，治疗组中生长卵泡和囊性卵泡的ERα表达高于对照组的生长卵泡。在卵泡内膜层，治疗组的三级卵泡中ERα表达显著更高。治疗组中检测到总ERα蛋白增加。在患有COD的动物中，所有生长、闭锁和囊性卵泡的颗粒细胞中ERβ表达较低，且该组中ERβ的总蛋白表达也较低。治疗动物的三级和囊性卵泡颗粒细胞层中PR的表达较低，且该组卵泡内膜层的免疫染色强度较弱。尽管通过蛋白质印迹法检测PR-B的表达没有差异，但治疗组中PR-A的表达较高，PR-C的表达较低。在囊性卵泡细胞中观察到强烈的HSP70免疫染色。通过蛋白质印迹法，检测到对照组卵巢样本中HSP70的蛋白表达高于治疗组。与对照动物相比，患有COD的动物卵巢表现出类固醇受体表达和亚型平衡的改变以及HSP70免疫表达增加。
