Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina; Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Consejo Nacional de Investigaciones Científicas y Tecnológicas, (CONICET), Argentina.
Res Vet Sci. 2013 Dec;95(3):1059-67. doi: 10.1016/j.rvsc.2013.07.012. Epub 2013 Aug 12.
Cystic ovarian disease (COD), which is considered one of the most important causes of reproductive failure in dairy cattle, induces intraovarian changes in the expression of numerous genes. The purpose of this study was to analyze the changes in the expression of Heat Shock Proteins (HSPs) in ovaries from bovines with cystic ovarian disease induced by ACTH. Immunoreactivity for Heat Shock Proteins (HSPs) in ovaries of cows with induced COD showed differential expression patterns in growing follicles from the control group. The immunopositive area for Hsp27 and Hsp60 in granulosa cells showed significant differences between tertiary follicles from normal cycling animals and those from animals with induced COD. The cysts showed increased Hsp27 immunostaining in theca cells in relation to tertiary follicles from normal cycling cows. Hsp70 immunostaining was more intense in cystic follicles than in other follicular categories from animals with induced COD, in both granulosa and theca cells. In granulosa cells, tertiary follicles from the control group showed higher levels of Hsp90 than cysts. These results demonstrate that there are differences in HSP protein expression when COD is induced. In fact, HSP expression would be part of the functional response to the changes in hormones and neurotransmitters induced by stress, indicating that HSPs can control hormonal functions and vice versa.
囊性卵巢疾病（COD）被认为是奶牛生殖失败的最重要原因之一，它会引起卵巢内许多基因表达的变化。本研究旨在分析由 ACTH 诱导的奶牛囊性卵巢疾病中热休克蛋白（HSPs）表达的变化。与对照组的生长卵泡相比，诱导 COD 奶牛卵巢中热休克蛋白（HSPs）的免疫反应显示出不同的表达模式。Hsp27 和 Hsp60 在颗粒细胞中的免疫阳性区域在正常循环动物和诱导 COD 动物的三级卵泡之间存在显著差异。与正常循环奶牛的三级卵泡相比，囊泡中的 Hsp27 免疫染色在卵泡膜细胞中增加。与诱导 COD 动物的其他卵泡类型相比，Hsp70 在囊泡中的免疫染色更强，无论是在颗粒细胞还是卵泡膜细胞中均如此。在颗粒细胞中，对照组的三级卵泡中的 Hsp90 水平高于囊泡。这些结果表明，在诱导 COD 时 HSP 蛋白表达存在差异。实际上，HSP 表达将是对由应激引起的激素和神经递质变化的功能反应的一部分，这表明 HSP 可以控制激素功能，反之亦然。
