Reproductive Medical Centre, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China.
Hubei Clinical Research Center for Assisted Reproductive Technology and Embryonic Development, Wuhan, 430060, People's Republic of China.
J Ovarian Res. 2019 Aug 9;12(1):74. doi: 10.1186/s13048-019-0548-7.
The mechanism of the pathological change of polycystic ovary syndrome (PCOS) is still unclear. Previous studies have shown that PCOS is a chronic nonspecific low-grade inflammatory condition, and that heat shock protein (HSP)70 has a potent anti-inflammatory property. So the aim of this study is to investigate the correlation between HSP70 and the hormones and inflammatory factors and to find out the role of HSP70 in the pathogenesis of PCOS.
Twenty female Sprague-Dawley (SD) rats (aged 23 days and weighted 80-90 g) were randomly divided into two groups (n = 10 per group), PCOS group and control group. PCOS group were subcutaneously injected with 6 mg/100 g dehydro-epiandrosterone (DHEA) for 20 consecutive days, the control group were subcutaneously injected with a solvent of equivalent amount. All the samples were collected in the morning fasting state, 12 h after the last administration. Histological examinations of ovarian tissues were analyzed. Hormone levels and inflammatory factors levels were measured by enzyme-linked immunosorbent assay (ELISA) kits.
Serum concentrations of testosterone (T) and luteinizing hormone (LH) were significantly higher in the PCOS group than the control group (P < 0.001), but the concentrations of estradiol (E), follicle stimulating hormone (FSH) and insulin didn't show significant difference between these two groups. All the concentrations of inflammatory factors including C-reactive protein (CRP), interleukin (IL)-6, IL-18, and tumor necrosis factor (TNF)-α. were significantly higher in PCOS group than the control group (P < 0.001). The expressions of HSP70 were significantly lower in serum but higher in ovarian tissues in the PCOS group than the control group. Spearman rank correlation analysis showed strong negative correlation of serum HSP70 levels with T, LH and all the detected inflammatory factors.
The abnormal expression of HSP70 correlated with testosterone and inflammatory factors, which indicates that HSP70 may play an important role in PCOS pathology.
多囊卵巢综合征（PCOS）的病理变化机制尚不清楚。既往研究表明，PCOS 是一种慢性非特异性低度炎症状态，热休克蛋白（HSP）70 具有很强的抗炎特性。因此，本研究旨在探讨 HSP70 与激素和炎症因子的相关性，以及 HSP70 在 PCOS 发病机制中的作用。
20 只 23 日龄、体重 80-90g 的雌性 Sprague-Dawley（SD）大鼠随机分为两组（每组 10 只），PCOS 组和对照组。PCOS 组连续 20 天皮下注射 6mg/100g 脱氢表雄酮（DHEA），对照组皮下注射等量溶剂。所有样本均在禁食 12 小时后，于最后一次给药的清晨采集。分析卵巢组织的组织学检查。采用酶联免疫吸附试验（ELISA）试剂盒检测激素和炎症因子水平。
PCOS 组血清睾酮（T）和黄体生成素（LH）浓度明显高于对照组（P<0.001），但雌二醇（E）、卵泡刺激素（FSH）和胰岛素浓度两组间无显著差异。PCOS 组所有炎症因子包括 C 反应蛋白（CRP）、白细胞介素（IL）-6、IL-18 和肿瘤坏死因子（TNF）-α的浓度均明显高于对照组（P<0.001）。PCOS 组血清 HSP70 表达明显低于对照组，卵巢组织中 HSP70 表达明显高于对照组。Spearman 秩相关分析显示，血清 HSP70 水平与 T、LH 和所有检测到的炎症因子呈强烈负相关。
HSP70 的异常表达与睾酮和炎症因子相关，表明 HSP70 可能在 PCOS 病理中发挥重要作用。
