Thompson M R, Forristal J, Kauffmann P, Madden T, Kozak K, Morris R E, Saelinger C B
Division of Digestive Diseases, University of Cincinnati College of Medicine, Ohio 45267-0524.
J Biol Chem. 1991 Feb 5;266(4):2390-6.
A Pseudomonas aeruginosa exotoxin A (PE) binding glycoprotein was affinity purified from toxin sensitive mouse LM cells. The binding protein was solubilized with Triton X-100 or Nonidet P-40 and purified on a PE-Sepharose affinity column. Polyacrylamide gel electrophoresis yielded a single band with an estimated molecular mass of greater than 300,000 Da. N-Linked carbohydrate was present, accounting for approximately 10% of the total mass of the molecule. The purified protein specifically bound PE. Incubation of purified protein specifically bound PE. Incubation of purified PE binding protein with toxin reduced toxicity to LM cells. We speculate on the role of this toxin binding glycoprotein in the intoxication process.
从对毒素敏感的小鼠LM细胞中亲和纯化出一种铜绿假单胞菌外毒素A（PE）结合糖蛋白。该结合蛋白用Triton X - 100或Nonidet P - 40溶解，并在PE - 琼脂糖亲和柱上纯化。聚丙烯酰胺凝胶电泳产生一条单一的带，估计分子量大于300,000 Da。存在N - 连接的碳水化合物，约占分子总质量的10%。纯化的蛋白特异性结合PE。纯化的PE结合蛋白与毒素一起孵育可降低对LM细胞的毒性。我们推测这种毒素结合糖蛋白在中毒过程中的作用。
