在特异性连接的烟酰胺腺嘌呤二核苷酸琼脂糖上对铜绿假单胞菌外毒素A进行亲和层析纯化。
Affinity chromatography purification of Pseudomonas aeruginosa exotoxin A on specifically linked NAD agarose.
作者信息
Cukor G, Nowak N A
出版信息
J Bacteriol. 1982 Mar;149(3):1162-5. doi: 10.1128/jb.149.3.1162-1165.1982.
Abstract
The specific binding of P. aeruginosa exotoxin A to NAD was exploited for the rapid purification of the toxin. Affinity chromatography on a column of agarose-N6-(aminohexyl)carbamoylmethyl-NAD resulted in an enzymatically, biologically, and immunologically active purified toxin preparation. Other NAD-agarose resins were not efficient substrates for toxin purification.
摘要
利用铜绿假单胞菌外毒素A与烟酰胺腺嘌呤二核苷酸（NAD）的特异性结合来快速纯化该毒素。在琼脂糖-N6-（氨基己基）氨甲酰甲基-NAD柱上进行亲和层析，得到了一种具有酶活性、生物学活性和免疫活性的纯化毒素制剂。其他NAD-琼脂糖树脂不是毒素纯化的有效底物。
相似文献
1
Affinity chromatography purification of Pseudomonas aeruginosa exotoxin A on specifically linked NAD agarose.在特异性连接的烟酰胺腺嘌呤二核苷酸琼脂糖上对铜绿假单胞菌外毒素A进行亲和层析纯化。
J Bacteriol. 1982 Mar;149(3):1162-5. doi: 10.1128/jb.149.3.1162-1165.1982.
2
[Isolation of Pseudomonas aeruginosa exotoxin A by affinity chromatography using specific antibodies].[利用特异性抗体通过亲和层析法分离铜绿假单胞菌外毒素A]
Zh Mikrobiol Epidemiol Immunobiol. 1989 Apr(4):64-8.
3
Preparation of Pseudomonas exotoxin A.绿脓杆菌外毒素A的制备
Methods Enzymol. 1988;165:147-52. doi: 10.1016/s0076-6879(88)65024-5.
4
Toxoids of Pseudomonas aeruginosa exotoxin-A: photoaffinity inactivation of purified toxin and purified toxin derivatives.铜绿假单胞菌外毒素A类毒素：纯化毒素及纯化毒素衍生物的光亲和失活
Infect Immun. 1984 Mar;43(3):1019-26. doi: 10.1128/iai.43.3.1019-1026.1984.
5
Investigation into the catalytic role for the tryptophan residues within domain III of Pseudomonas aeruginosa exotoxin A.铜绿假单胞菌外毒素A结构域III中色氨酸残基的催化作用研究。
Biochemistry. 1996 Dec 3;35(48):15134-42. doi: 10.1021/bi961985t.
6
[Immunoenzyme method of determining Pseudomonas aeruginosa exotoxin A by using a conjugate of beta-lactamase with specific antibodies].[利用β-内酰胺酶与特异性抗体的缀合物测定铜绿假单胞菌外毒素A的免疫酶法]
Antibiot Med Biotekhnol. 1987 Sep;32(9):693-6.
7
Diphtheria toxin and Pseudomonas aeruginosa exotoxin A: active-site structure and enzymic mechanism.白喉毒素与铜绿假单胞菌外毒素A：活性位点结构与酶促机制
Curr Top Microbiol Immunol. 1992;175:27-41. doi: 10.1007/978-3-642-76966-5_2.
8
Isolation and characterization of Pseudomonas aeruginosa exotoxin A binding glycoprotein from mouse LM cells.从小鼠LM细胞中分离和鉴定铜绿假单胞菌外毒素A结合糖蛋白。
J Biol Chem. 1991 Feb 5;266(4):2390-6.
9
Pseudomonas aeruginosa exotoxin A: effects of mutating tyrosine-470 and tyrosine-481 to phenylalanine.铜绿假单胞菌外毒素A：将酪氨酸-470和酪氨酸-481突变为苯丙氨酸的影响。
Biochemistry. 1988 Oct 4;27(20):7629-32. doi: 10.1021/bi00420a009.
10
Conformational integrity of a recombinant toxoid of Pseudomonas aeruginosa exotoxin A containing a deletion of glutamic acid-553.
Biochim Biophys Acta. 1992 Feb 14;1138(2):162-6. doi: 10.1016/0925-4439(92)90057-t.
引用本文的文献
1
Global discovery of protein kinases and other nucleotide-binding proteins by mass spectrometry.通过质谱法对蛋白激酶和其他核苷酸结合蛋白的全球发现。
Mass Spectrom Rev. 2016 Sep;35(5):601-19. doi: 10.1002/mas.21447. Epub 2014 Nov 5.
2
Comparative analysis of serum antibody responses to Pseudomonas aeruginosa exotoxin A by cystic fibrosis and intensive care unit patients.囊性纤维化患者与重症监护病房患者对铜绿假单胞菌外毒素A血清抗体反应的比较分析。
J Clin Microbiol. 1983 Sep;18(3):457-62. doi: 10.1128/jcm.18.3.457-462.1983.
本文引用的文献
1
Corneal infections in mice with toxin A and elastase mutants of Pseudomonas aeruginosa.铜绿假单胞菌毒素A和弹性蛋白酶突变体小鼠的角膜感染
J Infect Dis. 1980 Oct;142(4):547-55. doi: 10.1093/infdis/142.4.547.
2
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
3
Affinity chromatography purification of diphtheria toxin.白喉毒素的亲和层析纯化
Biotechnol Bioeng. 1974 Jul;16(7):925-31. doi: 10.1002/bit.260160706.
4
General ligands in affinity chromatography. Cofactor-substrate elution of enzymes bound to the immobilized nucleotides adenosine 5'-monophosphate and nicotinamide-adenine dinucleotide.亲和色谱中的通用配体。固定化核苷酸5'-单磷酸腺苷和烟酰胺腺嘌呤二核苷酸结合的酶的辅因子-底物洗脱。
Biochem J. 1972 May;127(4):625-31. doi: 10.1042/bj1270625.
5
Exotoxins of Pseudomonas aeruginosa. I. Factors that influence the production of exotoxin A.铜绿假单胞菌的外毒素。I. 影响外毒素A产生的因素。
J Infect Dis. 1973 Oct;128(4):506-13. doi: 10.1093/infdis/128.4.506.
6
Pseudomonas aeruginosa exotoxin: purification by preparative polyacrylamide gel electrophoresis and the development of a highly specific antitoxin serum.铜绿假单胞菌外毒素：通过制备性聚丙烯酰胺凝胶电泳进行纯化及高效特异性抗毒素血清的研制
Infect Immun. 1976 Jul;14(1):55-61. doi: 10.1128/iai.14.1.55-61.1976.
7
Chimeric toxins: toxic, disulfide-linked conjugate of concanavalin A with fragment A from diphtheria toxin.
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5319-23. doi: 10.1073/pnas.75.11.5319.
8
Toxin inhibitors of protein synthesis: production, purification, and assay of Pseudomonas aeruginosa toxin A.蛋白质合成的毒素抑制剂：铜绿假单胞菌毒素A的生产、纯化及测定
Methods Enzymol. 1979;60:780-93. doi: 10.1016/s0076-6879(79)60071-x.
9
Enzymatically active peptide from the adenosine diphosphate-ribosylating toxin of Pseudomonas aeruginosa.源自铜绿假单胞菌二磷酸腺苷核糖基化毒素的具有酶活性的肽。
Infect Immun. 1977 Jun;16(3):832-41. doi: 10.1128/iai.16.3.832-841.1977.
10
Large-scale purification and characterization of the exotoxin of Pseudomonas aeruginosa.铜绿假单胞菌外毒素的大规模纯化与特性分析
Infect Immun. 1976 Oct;14(4):1077-86. doi: 10.1128/iai.14.4.1077-1086.1976.
Zotero 插件