用于区分烟曲霉、黄曲霉、黑曲霉和土曲霉的多重聚合酶链反应。
Multiplex PCR for the discrimination of A. fumigatus, A. flavus, A. niger and A. terreus.
作者信息
Logotheti M, Kotsovili-Tseleni A, Arsenis G, Legakis N I
机构信息
Department of Microbiology, Medical School, University of Athens, Greece.
出版信息
J Microbiol Methods. 2009 Feb;76(2):209-11. doi: 10.1016/j.mimet.2008.10.006. Epub 2008 Oct 27.
Aspergillus pathogens usually infect immunocompromised patients with lethal outcome. We report a multiplex PCR assay for the discrimination of the most frequent Aspergillus pathogens, A. fumigatus, A. flavus, A. niger and A. terreus, through distinct amplicons of 250 bp, 200 bp, 150 bp and 450 bp respectively, derived from the rDNA gene of A. terreus and the aspergillopepsin genes of the remaining species.
曲霉病原体通常感染免疫功能低下的患者，预后往往致命。我们报告了一种多重PCR检测方法，用于区分最常见的曲霉病原体，即烟曲霉、黄曲霉、黑曲霉和土曲霉，分别通过250 bp、200 bp、150 bp和450 bp的不同扩增子进行区分，这些扩增子分别来自土曲霉的rDNA基因和其余物种的曲霉胃蛋白酶基因。
Zotero 插件