Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Fritz-Pregl-Straße 3, 6020 Innsbruck, Austria.
Can J Microbiol. 2011 Sep;57(9):765-8. doi: 10.1139/w11-064. Epub 2011 Aug 23.
The MycAssay™ Aspergillus real-time PCR kit was tested on tissues from patients with invasive fungal infections. Tissue samples from nine organ transplant recipients and 33 patients with haematological malignancy were from lung (n = 30), skin (n = 4), and others. Samples were preprocessed with proteinase K and lyticase, followed by DNA extraction and real-time PCR. For all samples, the sensitivity of the MycAssay Aspergillus test was 82% and specificity 79% relative to microscopy and 90% and 64%, respectively, compared with Aspergillus culture. The positive predictive value and negative predictive values compared with culture were 69% and 88% and were 88% and 69% compared with microscopy, respectively. The MycAssay Aspergillus test detected tissue invasive infections with Aspergillus fumigatus , Aspergillus flavus , and Aspergillus terreus.
MycAssay™ 曲霉实时 PCR 试剂盒在侵袭性真菌感染患者的组织中进行了测试。来自 9 名器官移植受者和 33 名血液恶性肿瘤患者的组织样本来自肺部（n = 30）、皮肤（n = 4）和其他部位。样品用蛋白酶 K 和溶菌酶预处理，然后进行 DNA 提取和实时 PCR。与显微镜相比，MycAssay 曲霉检测对所有样本的灵敏度分别为 82%和 79%，与曲霉培养相比，灵敏度分别为 90%和 64%。与培养相比，阳性预测值和阴性预测值分别为 69%和 88%，与显微镜相比，阳性预测值和阴性预测值分别为 88%和 69%。MycAssay 曲霉检测检测到烟曲霉、黄曲霉和土曲霉引起的组织侵袭性感染。
