Kobeticová Klára, Hofman Jakub, Holoubek Ivan
Research Centre for Environmental Chemistry and Ecotoxicology (RECETOX), Faculty of Science, Masaryk University, Kamenice 126/3, Brno CZ-62500, Czech Republic.
Environ Pollut. 2009 Feb;157(2):704-6. doi: 10.1016/j.envpol.2008.09.032. Epub 2008 Nov 7.
In this study, avoidance response of Enchytraeus albidus to LUFA 2.2 soil contaminated with pesticide carbendazim was investigated. The aim was to clarify minimal test duration and temporal changes in avoidance response due to contamination ageing. Firstly, the concentration causing 50% avoidance (EC(50)) was determined as 7.6 mg/kg. Then, test duration needed to reach this value (ET(50)=approximately 18 h) was identified. Finally, the capability of E. albidus avoidance test to reflect the changes of pollutant bioavailability was tested. The soil was spiked with carbendazim at the EC(50) concentration 1, 14, or 28 days before the test started and avoidance effects of fresh versus aged contamination were compared. The results indicated that enchytraeids preferred soil contaminated for 28 days prior to assay where carbendazim was probably less bioavailable than in freshly spiked soil. Our results open an interesting research area of potential use of avoidance tests for contaminant bioavailability assessment.
在本研究中，研究了白线蚓对受多菌灵污染的LUFA 2.2土壤的回避反应。目的是明确最小测试持续时间以及由于污染老化导致的回避反应的时间变化。首先，确定引起50%回避率的浓度（EC(50)）为7.6毫克/千克。然后，确定达到该值所需的测试持续时间（ET(50)约为18小时）。最后，测试了白线蚓回避试验反映污染物生物有效性变化的能力。在试验开始前1、14或28天，以EC(50)浓度向土壤中添加多菌灵，并比较新鲜污染与老化污染的回避效果。结果表明，在试验前28天受污染的土壤中，白线蚓更偏好于这种土壤，因为在这种土壤中多菌灵的生物有效性可能低于新添加多菌灵的土壤。我们的研究结果开启了一个有趣的研究领域，即回避试验在污染物生物有效性评估中的潜在应用。
