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胰腺腺泡细胞中依赖Ca2(+)的淀粉酶分泌在不激活与磷脂酶C相连的G蛋白的情况下发生。

Ca2(+)-dependent amylase secretion from pancreatic acinar cells occurs without activation of phospholipase C linked G-proteins.

作者信息

Padfield P J, Ding T G, Jamieson J D

机构信息

Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510.

出版信息

Biochem Biophys Res Commun. 1991 Jan 31;174(2):536-41. doi: 10.1016/0006-291x(91)91450-q.

DOI:10.1016/0006-291x(91)91450-q
PMID:1899567
Abstract

We have examined the influence of guanine nucleotides on Ca2(+)-dependent amylase secretion from SLO permeabilized rat pancreatic acini. GTP gamma S (100 microM) stimulated Ca2+ dependent amylase release, decreasing the EC50 for Ca2+ from 1.4 to 0.8 microM. By contrast, GDP (1mM) and dGDP (1mM) inhibited the maximal Ca2(+)-dependent secretory response. Measurement of IP3 liberation showed that Ca2+ stimulation did not increase the activity of phospholipase C (PLC) postulated to be linked to a G-protein termed Gp; GDP and dGDP must therefore be exerting their inhibitory action via a GTP-binding protein distinct from the PLC-linked Gp.

摘要

我们已经研究了鸟嘌呤核苷酸对经链球菌溶血素O（SLO）通透处理的大鼠胰腺腺泡细胞中Ca2+依赖的淀粉酶分泌的影响。GTPγS（100微摩尔）刺激了Ca2+依赖的淀粉酶释放，使Ca2+的半数有效浓度（EC50）从1.4微摩尔降至0.8微摩尔。相比之下，GDP（1毫摩尔）和dGDP（1毫摩尔）抑制了最大的Ca2+依赖的分泌反应。肌醇三磷酸（IP3）释放的测量结果表明，Ca2+刺激并没有增加假定与一种称为Gp的G蛋白相关联的磷脂酶C（PLC）的活性；因此，GDP和dGDP必定是通过一种不同于与PLC相关联的Gp的GTP结合蛋白发挥其抑制作用的。

相似文献

1
Ca2(+)-dependent amylase secretion from pancreatic acinar cells occurs without activation of phospholipase C linked G-proteins.胰腺腺泡细胞中依赖Ca2(+)的淀粉酶分泌在不激活与磷脂酶C相连的G蛋白的情况下发生。
Biochem Biophys Res Commun. 1991 Jan 31;174(2):536-41. doi: 10.1016/0006-291x(91)91450-q.
2
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引用本文的文献

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2
Cholecystokinin octapeptide inhibits Ca2+-dependent amylase secretion from permeabilized pancreatic acini by blocking the MgATP-dependent priming of exocytosis.胆囊收缩素八肽通过阻断胞吐作用的MgATP依赖性启动来抑制通透化胰腺腺泡细胞中Ca2+依赖性淀粉酶的分泌。
Biochem J. 1998 Feb 15;330 ( Pt 1)(Pt 1):329-34. doi: 10.1042/bj3300329.
3
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4
Differential effects of G-protein activators on 5-hydroxytryptamine and platelet-derived growth factor release from streptolysin-O-permeabilized human platelets.G蛋白激活剂对经链球菌溶血素O通透处理的人血小板释放5-羟色胺和血小板衍生生长因子的不同作用。
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