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蛋白激酶A调节通透化大鼠胰腺腺泡中钙和蛋白激酶C依赖性淀粉酶的释放。

Protein kinase A modulates Ca(2+)- and protein kinase C-dependent amylase release in permeabilized rat pancreatic acini.

作者信息

O'Sullivan A J, Jamieson J D

机构信息

Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510.

出版信息

Biochem J. 1992 Oct 15;287 ( Pt 2)(Pt 2):403-6. doi: 10.1042/bj2870403.

DOI:10.1042/bj2870403
PMID:1280101
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1133179/
Abstract

The role of protein kinase A (PKA) in the release of amylase from permeabilized pancreatic acini was investigated. Addition of cyclic AMP (cAMP) to permeabilized acini resulted in a potentiation of Ca(2+)-dependent amylase release, shifting the Ca2+ dose/response curve leftwards. As with protein kinase C (PKC) activation, this is due to an increase in the time of active discharge. The effect of cAMP was shown to be blocked by two inhibitors of PKA, H89 and the PKI-(5-24)-peptide. At low concentration, cAMP synergizes from phorbol 12-myristate 13-acetate (PMA), while at optimal concentrations cAMP and PMA are additive. PKA and PKC appear to work via similar, but not identical mechanisms.

摘要

研究了蛋白激酶A（PKA）在通透化胰腺腺泡淀粉酶释放中的作用。向通透化腺泡中添加环磷酸腺苷（cAMP）可增强Ca(2+)依赖性淀粉酶释放，使Ca2+剂量/反应曲线向左移动。与蛋白激酶C（PKC）激活一样，这是由于主动分泌时间增加所致。cAMP的作用被两种PKA抑制剂H89和PKI-(5-24)-肽阻断。在低浓度时，cAMP与佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）协同作用，而在最佳浓度时，cAMP和PMA具有加和性。PKA和PKC似乎通过相似但不完全相同的机制发挥作用。

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本文引用的文献

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The exocrine pancreas: the role of secretagogues, cyclic nucleotides, and calcium in enzyme secretion.外分泌胰腺：促分泌素、环核苷酸和钙在酶分泌中的作用。
Annu Rev Physiol. 1980;42:127-56. doi: 10.1146/annurev.ph.42.030180.001015.
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Synchronous exocytosis in Paramecium cells involves very rapid (less than or equal to 1 s), reversible dephosphorylation of a 65-kD phosphoprotein in exocytosis-competent strains.草履虫细胞中的同步胞吐作用涉及到在具备胞吐能力的菌株中，一种65-kD磷蛋白非常快速（小于或等于1秒）的可逆去磷酸化。
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Phosphorylation of low molecular mass cytosolic proteins by protein kinase C and protein kinase A in the rabbit exocrine pancreas.兔外分泌胰腺中蛋白激酶C和蛋白激酶A对低分子量胞质蛋白的磷酸化作用
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Biochem J. 1988 Dec 15;256(3):867-71. doi: 10.1042/bj2560867.
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Inhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a newly synthesized selective inhibitor of cyclic AMP-dependent protein kinase, N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), of PC12D pheochromocytoma cells.新型环磷酸腺苷依赖性蛋白激酶选择性抑制剂N-[2-(对溴肉桂氨基)乙基]-5-异喹啉磺酰胺（H-89）对福斯高林诱导的PC12D嗜铬细胞瘤细胞神经突生长和蛋白质磷酸化的抑制作用
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