Zolkov Chen, Avnir David, Armon Robert
Faculty of Civil and Environmental Engineering, Division of Environmental, Water and Agricultural Engineering, Technion, Haifa 32000, Israel.
J Virol Methods. 2009 Feb;155(2):132-5. doi: 10.1016/j.jviromet.2008.09.031. Epub 2008 Nov 21.
Hybrid sol-gel films were used to grow Buffalo Green Monkey kidney cell tissues, which were used for poliovirus-1 detection. It is shown that the sol-gel approach allows cutting the standard EPA procedure from 48 to 24h of detection time; that better visualization of the plaques is obtained; that a variety of stains, including fluorescence, can be used; and that the shelf life of the resulting plaques system is well over a year.