水牛绿猴肾细胞摇瓶培养的发展：在肠道病毒检测中的潜在应用

Development of a shaker culture of Buffalo green monkey kidney cells: potential use for detection of enteroviruses.

作者信息

Goldstein G, Guskey L E

出版信息

Appl Environ Microbiol. 1982 Aug;44(2):317-20. doi: 10.1128/aem.44.2.317-320.1982.

DOI:10.1128/aem.44.2.317-320.1982

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC242012/

Abstract

Buffalo green monkey kidney cells were adapted to grow as shaker cultures. Replication of environmental and clinical isolates of poliovirus, coxsackievirus, and echovirus in these cultures was analyzed by plaque assay and compared with replication in Buffalo green monkey kidney cell monolayers and HEp-2 cell shaker cultures. Dose-response tests with various concentrations of Mahoney type 1 poliovirus indicated that Buffalo green monkey kidney cell shaker cultures could detect as little as 1 PFU in an inoculum of 0.2 ml. These data suggest that Buffalo green monkey kidney cell shaker cultures can be effectively used for the detection of small quantities of enteroviruses from environmental sources.

摘要

水牛绿猴肾细胞经适应后可在摇瓶培养中生长。通过空斑试验分析了脊髓灰质炎病毒、柯萨奇病毒和埃可病毒的环境分离株和临床分离株在这些培养物中的复制情况，并与它们在水牛绿猴肾细胞单层培养物和人喉上皮癌细胞（HEp-2）摇瓶培养物中的复制情况进行了比较。用不同浓度的1型马奥尼株脊髓灰质炎病毒进行剂量反应试验表明，在0.2 ml接种物中，水牛绿猴肾细胞摇瓶培养物能检测到低至1个空斑形成单位（PFU）。这些数据表明，水牛绿猴肾细胞摇瓶培养物可有效地用于检测环境来源的少量肠道病毒。

相似文献

1

Development of a shaker culture of Buffalo green monkey kidney cells: potential use for detection of enteroviruses.

Appl Environ Microbiol. 1982 Aug;44(2):317-20. doi: 10.1128/aem.44.2.317-320.1982.

2

Comparison of cell cultures for rapid isolation of enteroviruses.

J Clin Microbiol. 1988 Dec;26(12):2576-80. doi: 10.1128/jcm.26.12.2576-2580.1988.

3

Susceptibility of the VERO line of African green monkey kidney cells to human enteroviruses.

J Hyg (Lond). 1974 Feb;72(1):23-30. doi: 10.1017/s0022172400023160.

4

Inhibitory action of N-2-hydroxyphenylthiourea derivatives on picornavirus multiplication in cell cultures.

Acta Virol. 1971 Sep;15(5):404-10.

5

Development and validation of an integrated cell culture-qRTPCR assay for simultaneous quantification of coxsackieviruses, echoviruses, and polioviruses in disinfection studies.

Water Sci Technol. 2010;61(2):375-87. doi: 10.2166/wst.2010.818.

6

Maintenance of primary African green monkey kidney (pAGMK) and Vero cells at room temperature (25 degrees). A system for virus isolation in community practice.

Can J Microbiol. 1974 Mar;20(3):391-7. doi: 10.1139/m74-060.

7

A microneutralization test for the identification of enterovirus isolates.

J Virol Methods. 1986 Nov;14(3-4):305-7. doi: 10.1016/0166-0934(86)90032-7.

8

[Effect of sodium salicylate on the "in vitro" development of RNA viruses (author's transl)].

Ann Microbiol (Paris). 1975 Apr;126(3):381-7.

9

Comparison of rhabdomyosarcoma, buffalo green monkey kidney epithelial, A549 (human lung epithelial) cells and human embryonic lung fibroblasts for isolation of enteroviruses from clinical samples.

J Clin Virol. 1998 Jul 24;11(1):61-5. doi: 10.1016/s0928-0197(98)00026-9.

10

Influence of inoculum size, incubation temperature, and cell culture density on virus detection in environmental samples.

Can J Microbiol. 1985 Nov;31(11):977-80. doi: 10.1139/m85-185.

引用本文的文献

1

Detection of poliovirus by ICC/qPCR in concentrated water samples has greater sensitivity and is less costly using BGM cells in suspension as compared to monolayers.

Virol J. 2010 Oct 25;7:282. doi: 10.1186/1743-422X-7-282.

本文引用的文献

1

Application of the most probable number method for estimating concentrations of animal viruses by the tissue culture technique.

Virology. 1958 Aug;6(1):27-42. doi: 10.1016/0042-6822(58)90057-6.

2

High yield growth and purification of human parainfluenza type 3 virus and initial analysis of viral structural proteins.

J Gen Virol. 1981 May;54(Pt 1):115-23. doi: 10.1099/0022-1317-54-1-115.

3

Estimation of low numbers of Escherichia coli bacteriophage by use of the most probable number method.

Appl Microbiol. 1966 Mar;14(2):141-4. doi: 10.1128/am.14.2.141-144.1966.

4

Characteristics of the BGM line of cells from African green monkey kidney. Brief report.

Arch Gesamte Virusforsch. 1970;32(4):389-92. doi: 10.1007/BF01250067.

5

BGM, a continuous cell line more sensitive than primary rhesus and African green kidney cells for the recovery of viruses from water.

Health Lab Sci. 1974 Oct;11(4):275-82.

6

Adaptation of BHK-21 cells to growth in shaker culture and subsequent challenge by Japanese encephalitis virus.

Appl Microbiol. 1975 Sep;30(3):433-8. doi: 10.1128/am.30.3.433-438.1975.

7

Characterization of an in vitro persistent-state measles virus infection: establishment and virological characterization of the BGM/MV cell line.

Infect Immun. 1975 Jan;11(1):152-8. doi: 10.1128/iai.11.1.152-158.1975.

8

Comparative sensitivity of various cell culture systems for isolation of viruses from wastewater and fecal samples.

Appl Environ Microbiol. 1978 Sep;36(3):480-6. doi: 10.1128/aem.36.3.480-486.1978.

9

Comparative sensitivity of the BGM cell line for isolation of enteric viruses.

Health Lab Sci. 1976 Apr;13(2):115-7.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。