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水牛绿猴肾细胞摇瓶培养的发展：在肠道病毒检测中的潜在应用

Development of a shaker culture of Buffalo green monkey kidney cells: potential use for detection of enteroviruses.

作者信息

Goldstein G, Guskey L E

出版信息

Appl Environ Microbiol. 1982 Aug;44(2):317-20. doi: 10.1128/aem.44.2.317-320.1982.

DOI:10.1128/aem.44.2.317-320.1982
PMID:6289745
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC242012/
Abstract

Buffalo green monkey kidney cells were adapted to grow as shaker cultures. Replication of environmental and clinical isolates of poliovirus, coxsackievirus, and echovirus in these cultures was analyzed by plaque assay and compared with replication in Buffalo green monkey kidney cell monolayers and HEp-2 cell shaker cultures. Dose-response tests with various concentrations of Mahoney type 1 poliovirus indicated that Buffalo green monkey kidney cell shaker cultures could detect as little as 1 PFU in an inoculum of 0.2 ml. These data suggest that Buffalo green monkey kidney cell shaker cultures can be effectively used for the detection of small quantities of enteroviruses from environmental sources.

摘要

水牛绿猴肾细胞经适应后可在摇瓶培养中生长。通过空斑试验分析了脊髓灰质炎病毒、柯萨奇病毒和埃可病毒的环境分离株和临床分离株在这些培养物中的复制情况，并与它们在水牛绿猴肾细胞单层培养物和人喉上皮癌细胞（HEp-2）摇瓶培养物中的复制情况进行了比较。用不同浓度的1型马奥尼株脊髓灰质炎病毒进行剂量反应试验表明，在0.2 ml接种物中，水牛绿猴肾细胞摇瓶培养物能检测到低至1个空斑形成单位（PFU）。这些数据表明，水牛绿猴肾细胞摇瓶培养物可有效地用于检测环境来源的少量肠道病毒。

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本文引用的文献

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Application of the most probable number method for estimating concentrations of animal viruses by the tissue culture technique.应用最大可能数法通过组织培养技术估计动物病毒浓度。
Virology. 1958 Aug;6(1):27-42. doi: 10.1016/0042-6822(58)90057-6.
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Estimation of low numbers of Escherichia coli bacteriophage by use of the most probable number method.使用最大可能数法估算少量大肠杆菌噬菌体
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BGM, a continuous cell line more sensitive than primary rhesus and African green kidney cells for the recovery of viruses from water.BGM是一种连续细胞系，在从水中分离病毒方面比恒河猴原代肾细胞和非洲绿猴肾细胞更敏感。
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Adaptation of BHK-21 cells to growth in shaker culture and subsequent challenge by Japanese encephalitis virus.将BHK - 21细胞适应摇瓶培养生长并随后用日本脑炎病毒进行攻击。
Appl Microbiol. 1975 Sep;30(3):433-8. doi: 10.1128/am.30.3.433-438.1975.
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Characterization of an in vitro persistent-state measles virus infection: establishment and virological characterization of the BGM/MV cell line.体外持续性麻疹病毒感染的特征：BGM/MV细胞系的建立及病毒学特征
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Comparative sensitivity of various cell culture systems for isolation of viruses from wastewater and fecal samples.用于从废水和粪便样本中分离病毒的各种细胞培养系统的比较敏感性。
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