Iwaniuk Andrew N, Marzban Hassan, Pakan Janelle M P, Watanabe Masahiko, Hawkes Richard, Wylie Douglas R W
Department of Psychology, University of Alberta, Edmonton, Alta., Canada.
J Chem Neuroanat. 2009 Jan;37(1):55-63. doi: 10.1016/j.jchemneu.2008.10.001. Epub 2008 Oct 18.
The parasagittal organization of the mammalian cerebellar cortex into zones has been well characterized by immunohistochemical, hodological and physiological studies in recent years. The pattern of these parasagittal bands across the cerebellum is highly conserved across mammals, but whether a similar conservation of immunohistochemically defined parasagittal bands occurs within birds has remained uncertain. Here, we examine the compartmentation of the cerebellar cortex of a group of birds with unique cerebellar morphology-hummingbirds (Trochilidae). Immunohistochemical techniques were used to characterize the expression of zebrin II (aldolase C) and phospholipase C beta 4 (PLC beta 4) in the cerebellar cortex of two hummingbird species. A series of zebrin II immunopositive/immunonegative parasagittal stripes was apparent across most folia representing three major transverse zones: an anterior zone with a central stripe flanked by three lateral stripes on either side; a central zone of high/low immunopositive stripes; and a posterior zone with a central stripe flanked by four to six lateral stripes on either side. In addition, both folia I and X were uniformly immunopositive. The pattern of PLC beta 4 immunoreactivity was largely complementary-PLC beta 4 positive stripes were zebrin II negative and vice versa. The similarity of zebrin II expression between the hummingbirds and the pigeon indicates that the neurochemical compartmentation of the cerebellar cortex in birds is highly conserved, but species differences in the number and width of stripes do occur.
近年来，免疫组织化学、神经束路学和生理学研究已充分阐明了哺乳动物小脑皮质矢状旁区的分区情况。这些矢状旁带在整个小脑中的分布模式在哺乳动物中高度保守，但鸟类体内是否存在类似的免疫组织化学定义的矢状旁带保守性仍不明确。在此，我们研究了一组具有独特小脑形态的鸟类——蜂鸟（蜂鸟科）的小脑皮质分区。免疫组织化学技术被用于表征两种蜂鸟物种小脑皮质中zebrin II（醛缩酶C）和磷脂酶Cβ4（PLCβ4）的表达。在大多数小叶中，一系列zebrin II免疫阳性/免疫阴性矢状旁条纹很明显，代表三个主要横向区域：一个前区，中央条纹两侧各有三条外侧条纹；一个高/低免疫阳性条纹的中区；以及一个后区，中央条纹两侧各有四到六条外侧条纹。此外，I小叶和X小叶均呈均匀免疫阳性。PLCβ4免疫反应性模式在很大程度上是互补的——PLCβ4阳性条纹是zebrin II阴性，反之亦然。蜂鸟和鸽子之间zebrin II表达的相似性表明，鸟类小脑皮质的神经化学分区高度保守，但条纹的数量和宽度确实存在物种差异。
