猪链球菌2型2-酮-3-脱氧-6-磷酸葡萄糖酸（KDPG）醛缩酶的克隆、表达、纯化、结晶及初步X射线衍射分析
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase from Streptococcus suis serotype 2.
作者信息
Zhang Qiangmin, Gao Feng, Qi Jianxun, Cheng Hao, Liu Yiwei, Gao George F
机构信息
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Nov 1;64(Pt 11):997-9. doi: 10.1107/S1744309108028340. Epub 2008 Oct 25.
Abstract
2-Keto-3-deoxy-6-phosphogluconate (KDPG) adolase from pathogenic Streptococcus suis serotype 2 was crystallized using the hanging-drop vapour-diffusion method at 291 K. X-ray diffraction data were collected to 2.8 A resolution. The crystal belonged to space group R32, with unit-cell parameters a = b = 126.4, c = 415.9 A, alpha = beta = 90, gamma = 120 degrees . Assuming the presence of six molecules in the asymmetric unit gave a V(M) value of 2.32 A(3) Da(-1) and a solvent content of 47.12%.
摘要
采用悬滴气相扩散法，在291 K下对致病性猪链球菌2型的2-酮-3-脱氧-6-磷酸葡萄糖酸（KDPG）醛缩酶进行了结晶。收集到了分辨率为2.8 Å的X射线衍射数据。晶体属于R32空间群，晶胞参数a = b = 126.4，c = 415.9 Å，α = β = 90°，γ = 120°。假设不对称单元中存在六个分子，则V(M)值为2.32 ų Da⁻¹，溶剂含量为47.12%。
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