从培养的黄连细胞中分离抗除草剂的4-羟基苯丙酮酸双加氧酶。
Isolation of herbicide-resistant 4-hydroxyphenylpyruvate dioxygenase from cultured Coptis japonica cells.
作者信息
Liang Yuling, Minami Hiromichi, Sato Fumihiko
机构信息
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, PR China.
出版信息
Biosci Biotechnol Biochem. 2008 Nov;72(11):3059-62. doi: 10.1271/bbb.80466. Epub 2008 Nov 7.
4-Hydroxyphenylpyruvate dioxygenase (HPPD) catalyzes the formation of homogentisate from 4-hydroxyphenylpyruvate and O(2). In plants, HPPD has been identified as a molecular target for herbicides. We report the isolation and characterization of a cDNA encoding a HPPD from cultured Coptis japonica cells. Recombinant CjHPPD showed significantly higher half-maximum inhibitory concentration (IC(50)) values for the HPPD-inhibiting herbicide destosyl pyrazolate than other plant HPPDs.
4-羟基苯丙酮酸双加氧酶（HPPD）催化4-羟基苯丙酮酸和O₂生成尿黑酸。在植物中，HPPD已被确定为除草剂的分子靶标。我们报道了从黄连培养细胞中分离和鉴定编码HPPD的cDNA。重组黄连HPPD对抑制HPPD的除草剂去甲苯磺酰唑草酯的半最大抑制浓度（IC₅₀）值显著高于其他植物HPPD。
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