Holaday J W, Meyerhoff J L, Natelson B H
Endocrinology. 1977 Apr;100(4):1178-85. doi: 10.1210/endo-100-4-1178.
Cortisol secretory patterns were studied in two chair-adapted rhesus monkeys by simultaneous measurement of plasma concentration and specific activity of cortisol after an iv bolus of 14C-labeled hormone. The results indicated that fluctuating plasma cortisol concentrations are the result of episodic secretion by the adrenal cortex. Specific activity changes during these spontaneous secretory bursts indicated occasional submaximal activity. In addition, cortisol secretory rates calculated during basal (20.4 mug/min) and ACTH-stimulated (28.4mug/min) conditions in a total of seven monkeys were significantly different (P less than 0.05), further demonstrating that spontaneous secretory bursts were usually sub-maximal. From plasma samples collected at 10 min intervals, a cortisol distribution t1/2 of 6 min and a clearance t1/2 of 66 min were found. The apparent volume of distribution for this hormone was 4.8 liters, a value far in excess of extracellular fluid volume estimates. The circadian pattern of plasma cortisol in these monkeys resembled that reported for man, but monkeys had twice as many episodic bursts and over twice the mean cortisol levels as man. However, the 24 h production rate was 10.5 mg, a value within the range of human production.
通过静脉注射14C标记的激素后，同时测量血浆中皮质醇的浓度和比活性，对两只适应椅子环境的恒河猴的皮质醇分泌模式进行了研究。结果表明，血浆皮质醇浓度的波动是肾上腺皮质阵发性分泌的结果。在这些自发分泌脉冲期间的比活性变化表明偶尔会出现次最大活性。此外，在总共七只猴子的基础状态（20.4微克/分钟）和促肾上腺皮质激素刺激状态（28.4微克/分钟）下计算的皮质醇分泌率有显著差异（P小于0.05），进一步证明自发分泌脉冲通常是次最大的。从每隔10分钟采集的血浆样本中，发现皮质醇的分布半衰期为6分钟，清除半衰期为66分钟。这种激素的表观分布容积为4.8升，该值远远超过细胞外液容积估计值。这些猴子血浆皮质醇的昼夜模式与人类报告的相似，但猴子的阵发性脉冲次数是人类的两倍，平均皮质醇水平是人类的两倍多。然而，24小时产生率为10.5毫克，该值在人类产生范围之内。
