Sarnyai Z, Veldhuis J D, Mello N K, Mendelson J H, Erös-Sarnyai M, Mercer G, Gelles H, Kelly M
Alcohol and Drug Abuse Research Center, Harvard Medical School, McLean Hospital, Belmont, Massachusetts 02178.
J Clin Endocrinol Metab. 1995 Jan;80(1):54-9. doi: 10.1210/jcem.80.1.7829639.
The ultradian release of ACTH and cortisol was investigated in six male rhesus monkeys (Macaca mulatta) with an intensive (2-min) blood-sampling procedure to investigate micropulsatile hormone secretory patterns. A sensitive and specific immunoradiometric assay was used to measure plasma ACTH concentrations. An objective pulse detection algorithm (Cluster) was used to assess the pulsatility of ACTH and cortisol release. The temporally coincident release of ACTH and cortisol was also examined. Venous blood samples were collected (over < 15 s) every 2 min for 120 min beginning at 1300 h. The number of ACTH peaks (3.2 peaks/h), interpulse intervals (19 +/- 2.4 min), and pulse amplitudes (9.7 +/- 1.6 pmol/L) in rhesus monkey were similar to corresponding measures of ACTH release in humans (3.3 peaks/h, 18 +/- 0.8 min, and 4.7 +/- 1.0 pmol/L, respectively). The number of cortisol peaks (2.3 peaks/h), interpulse interval (26 +/- 8.6 min), and other characteristics of pulsatile cortisol release were also determined. There was a 32.4% exact concordance of ACTH with cortisol peaks (11 of 34; P < 0.001). Fifty-six percent of ACTH peaks (19 of 34) were followed by a cortisol peak within 10 min (P < 0.02). There was a significant correlation between the ACTH and coincident cortisol pulse amplitudes (P < 0.0001). The amplitudes of ACTH peaks coincident with cortisol peaks at 0 min time lag were significantly higher than ACTH peaks not temporally coupled with cortisol peaks. Our data indicate that 1) high frequency, low amplitude micropulsatile ACTH secretion in rhesus monkeys is very similar to the high frequency ACTH rhythm in humans; 2) temporally concordant ACTH and cortisol release episodes may be amplitude coupled; and 3) an adequate incremental ACTH pulse amplitude may elicit a concurrent cortisol release episode from the adrenal cortex. These data suggest that the rhesus monkey is a potentially useful model for the study of neuroendocrine control of ACTH release.
采用密集（2分钟）采血程序，对6只雄性恒河猴（猕猴）促肾上腺皮质激素（ACTH）和皮质醇的超日节律释放进行了研究，以探究微量脉冲式激素分泌模式。使用灵敏且特异的免疫放射分析方法测定血浆ACTH浓度。采用一种客观的脉冲检测算法（聚类算法）评估ACTH和皮质醇释放的脉冲性。还对ACTH和皮质醇的同步释放情况进行了检查。从13:00开始，每2分钟采集一次静脉血样本（采集时间<15秒），共采集120分钟。恒河猴的ACTH峰值数量（3.2个峰值/小时）、脉冲间期（19±2.4分钟）和脉冲幅度（9.7±1.6皮摩尔/升）与人类ACTH释放的相应测量值相似（分别为3.3个峰值/小时、18±0.8分钟和4.7±1.0皮摩尔/升）。还确定了皮质醇峰值数量（2.3个峰值/小时）、脉冲间期（26±8.6分钟）以及脉冲式皮质醇释放的其他特征。ACTH峰值与皮质醇峰值的精确一致性为32.4%（34个中有11个；P<0.001）。56%的ACTH峰值（34个中有19个）之后在10分钟内出现皮质醇峰值（P<0.02）。ACTH与同步的皮质醇脉冲幅度之间存在显著相关性（P<0.0001）。在0分钟时滞与皮质醇峰值同步的ACTH峰值幅度显著高于与皮质醇峰值无时间关联的ACTH峰值。我们的数据表明：1）恒河猴高频、低幅度的微量脉冲式ACTH分泌与人类的高频ACTH节律非常相似；2）时间上同步的ACTH和皮质醇释放事件可能在幅度上相互关联；3）足够的ACTH脉冲幅度增量可能引发肾上腺皮质同时释放皮质醇。这些数据表明，恒河猴是研究ACTH释放的神经内分泌控制的一个潜在有用模型。
