Kase Satoru, Parikh Jignesh G, Youssef Peter N, Murphree A Linn, Rao Narsing A
Doheny Eye Institute, Los Angeles, CA 90033, USA.
Arch Ophthalmol. 2008 Nov;126(11):1539-42. doi: 10.1001/archopht.126.11.1539.
To analyze the histopathology and expression of transforming growth factor beta (TGF-beta) in retinoblastoma with and without cataractous changes.
Twenty patients with unilateral retinoblastoma underwent enucleation. None of these patients had received preoperative chemotherapy or radiotherapy. Formalin-fixed, paraffin-embedded tissue sections were examined histologically for the presence of morgagnian globules or liquefaction of lens fibers; TGF-beta was immunolocalized using an anti-TGF-beta antibody.
Two globes showed several morgagnian globules and liquefaction of the lens fibers, representing cataractous changes. One patient had posterior subcapsular cataract; the other, anterior polar cataract. In both cases, prominent cytoplasmic immunoreactivity for TGF-beta was detected in retinoblastoma cells. In contrast, 3 patients showed histologic evidence of minor cataractous changes. The globes with either minor or no cataractous changes revealed minimal to no expression of TGF-beta.
These results suggest that TGF-beta produced by retinoblastoma cells may induce cataract formation. Clinical Relevance The growth factors produced by retinoblastoma cells may lead to associated pathologies, such as cataracts, in the ocular structures. This study implies that when a child presents with a unilateral cataract, retinoblastoma should be excluded as the primary diagnosis.
分析伴有和不伴有白内障改变的视网膜母细胞瘤的组织病理学及转化生长因子β（TGF-β）的表达情况。
20例单侧视网膜母细胞瘤患者接受了眼球摘除术。这些患者均未接受过术前化疗或放疗。对福尔马林固定、石蜡包埋的组织切片进行组织学检查，以确定是否存在莫尔加尼球或晶状体纤维液化；使用抗TGF-β抗体对TGF-β进行免疫定位。
2个眼球显示有多个莫尔加尼球和晶状体纤维液化，代表白内障改变。1例患者有后囊下白内障；另1例有前极性白内障。在这两种情况下，视网膜母细胞瘤细胞中均检测到TGF-β显著的细胞质免疫反应性。相比之下，3例患者显示有轻微白内障改变的组织学证据。有轻微或无白内障改变的眼球显示TGF-β表达极少或无表达。
这些结果表明，视网膜母细胞瘤细胞产生的TGF-β可能诱导白内障形成。临床意义视网膜母细胞瘤细胞产生的生长因子可能导致眼部结构出现相关病变，如白内障。本研究提示，当儿童出现单侧白内障时，应排除视网膜母细胞瘤作为主要诊断。
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