Orellana Maria Eugenia, Quezada Carlos, Maloney Shawn C, Antecka Emilia, Balazsi Matthew, Burnier Miguel N
Instituto Anatomopatológico 'Dr. José A. O'Daly', Universidad Central de Venezuela, Caracas, Venezuela.
Ophthalmic Res. 2015;53(2):100-8. doi: 10.1159/000368718. Epub 2015 Feb 5.
SIRT2 and SIRT6 are members of the sirtuin family and are associated with cancer development and progression in certain tumours, but their expression in retinoblastoma has not been studied. The primary objective of our study was to determine the expression of SIRT2 and SIRT6 in human retinoblastoma cases.
Eighteen formalin-fixed paraffin-embedded blocks of retinoblastoma cases from the Ocular Pathology Registry at the Henry C. Witelson Ocular Pathology Laboratory were obtained, classified and immunostained for SIRT2 and SIRT6 using mouse monoclonal antibodies.
Sixteen cases were poorly differentiated retinoblastoma cases. SIRT2 and SIRT6 were expressed in all cases of retinoblastoma although differences in the staining intensity were found between cases. SIRT2 and SIRT6 expression was also observed in various normal structures of the remaining ocular tissue.
SIRT2 and SIRT6 are expressed in retinoblastoma, as well as in some normal ocular structures. While precise roles of these proteins must still be determined in retinoblastoma, their expression profiles suggest that further functional studies of both SIRT2 and SIRT6 should be pursued in this cancer.
SIRT2和SIRT6是沉默调节蛋白家族的成员，与某些肿瘤的发生发展相关，但它们在视网膜母细胞瘤中的表达尚未得到研究。我们研究的主要目的是确定SIRT2和SIRT6在人类视网膜母细胞瘤病例中的表达情况。
从亨利·C·维特森眼病理实验室的眼病理登记处获取了18个视网膜母细胞瘤病例的福尔马林固定石蜡包埋块，进行分类并用小鼠单克隆抗体对SIRT2和SIRT6进行免疫染色。
16例为低分化视网膜母细胞瘤病例。所有视网膜母细胞瘤病例均表达SIRT2和SIRT6，尽管病例之间染色强度存在差异。在剩余眼组织的各种正常结构中也观察到了SIRT2和SIRT6的表达。
SIRT2和SIRT6在视网膜母细胞瘤以及一些正常眼结构中均有表达。虽然这些蛋白在视网膜母细胞瘤中的具体作用仍有待确定，但它们的表达谱表明，应在这种癌症中对SIRT2和SIRT6进行进一步的功能研究。
