去甲氧基-Q6的羟基化作用是酵母辅酶Q6生物合成中的一个控制点。
Hydroxylation of demethoxy-Q6 constitutes a control point in yeast coenzyme Q6 biosynthesis.
作者信息
Padilla S, Tran U C, Jiménez-Hidalgo M, López-Martín J M, Martín-Montalvo A, Clarke C F, Navas P, Santos-Ocaña C
机构信息
Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-CSIC and Centre for Biomedical Research on Rare Diseases (CIBERER), Carretera de Utrera, km 1, ISCIII, 41013 Sevilla, Spain.
出版信息
Cell Mol Life Sci. 2009 Jan;66(1):173-86. doi: 10.1007/s00018-008-8547-7.
Coenzyme Q is a lipid molecule required for respiration and antioxidant protection. Q biosynthesis in Saccharomyces cerevisiae requires nine proteins (Coq1p-Coq9p). We demonstrate in this study that Q levels are modulated during growth by its conversion from demethoxy-Q (DMQ), a late intermediate. Similar conversion was produced when cells were subjected to oxidative stress conditions. Changes in Q(6)/DMQ(6) ratio were accompanied by changes in COQ7 gene mRNA levels encoding the protein responsible for the DMQ hydroxylation, the penultimate step in Q biosynthesis pathway. Yeast coq null mutant failed to accumulate any Q late biosynthetic intermediate. However, in coq7 mutants the addition of exogenous Q produces the DMQ synthesis. Similar effect was produced by over-expressing ABC1/COQ8. These results support the existence of a biosynthetic complex that allows the DMQ(6) accumulation and suggest that Coq7p is a control point for the Q biosynthesis regulation in yeast.
辅酶Q是呼吸作用和抗氧化保护所需的脂质分子。酿酒酵母中的Q生物合成需要九种蛋白质（Coq1p-Coq9p）。我们在本研究中证明，在生长过程中，Q的水平通过其从脱甲氧基-Q（DMQ）（一种晚期中间体）的转化而受到调节。当细胞受到氧化应激条件时，也会产生类似的转化。Q(6)/DMQ(6)比值的变化伴随着编码负责DMQ羟基化（Q生物合成途径中的倒数第二步）的蛋白质的COQ7基因mRNA水平的变化。酵母coq null突变体无法积累任何Q晚期生物合成中间体。然而，在coq7突变体中，添加外源Q会产生DMQ合成。过表达ABC1/COQ8也产生了类似的效果。这些结果支持存在一种允许DMQ(6)积累的生物合成复合物，并表明Coq7p是酵母中Q生物合成调控的一个控制点。
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