Marres C A, de Vries S, Grivell L A
Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.
Eur J Biochem. 1991 Feb 14;195(3):857-62. doi: 10.1111/j.1432-1033.1991.tb15775.x.
We have recently described the isolation of a mitochondrial rotenone-insensitive NADH:ubiquinone oxidoreductase from Saccharomyces cerevisiae [de Vries, S. & Grivell, L. A. (1988) Eur. J. Biochem. 176, 377-384]. We now report the isolation of the nuclear gene encoding this single-subunit enzyme. Null mutants have been constructed by means of one-step gene disruption. Oxygen-uptake experiments, performed with mitochondria isolated from the mutant cells, showed that this NADH dehydrogenase catalyzes the oxidation of NADH generated inside the mitochondrion. Inactivation of this NADH dehydrogenase does not affect growth on glucose and ethanol, but growth on lactate, pyruvate and acetate is impaired or absent. This phenotype is discussed in terms of the interplay between different metabolic pathways in yeast.
我们最近描述了从酿酒酵母中分离出一种对鱼藤酮不敏感的线粒体NADH：泛醌氧化还原酶[德弗里斯，S. & 格里维尔，L. A.（1988年）《欧洲生物化学杂志》176，377 - 384]。我们现在报告编码这种单亚基酶的核基因的分离情况。通过一步基因破坏构建了缺失突变体。用从突变细胞中分离出的线粒体进行的氧摄取实验表明，这种NADH脱氢酶催化线粒体内产生的NADH的氧化。这种NADH脱氢酶的失活并不影响在葡萄糖和乙醇上的生长，但在乳酸、丙酮酸和乙酸上的生长受到损害或无法进行。根据酵母中不同代谢途径之间的相互作用对这种表型进行了讨论。
