Sodré Cátia Lacerda, Chapeaurouge Alexander D, Kalume Dário Eluan, de Mendonça Lima Leila, Perales Jonas, Fernandes Octavio
Laboratory of Molecular Epidemiology of Infectious Diseases, Oswaldo Cruz Institute-IOC, Oswaldo Cruz Foundation-FIOCRUZ. Av. Brasil, 4365-Manguinhos, Rio de Janeiro, RJ 21045-900, Brazi.
Arch Microbiol. 2009 Feb;191(2):177-84. doi: 10.1007/s00203-008-0439-6. Epub 2008 Nov 11.
In this work two-dimensional gel electrophoresis combined with mass spectrometry was carried out in order to start the construction of a map of soluble proteins from epimastigote form of Trypanosoma cruzi CL Brener. This strain is a hybrid organism derived from two genotypes, T. cruzi I and T. cruzi II and was chosen for genome sequencing. The two-dimensional gel electrophoresis showed that most of proteins focused at 4-7 pH range. The identification demonstrated that several proteins were in multiple isoforms, such as tubulin and heat shock proteins. Potential targets for development of chemotherapeutic agents like arginine kinase, an enzyme absent from mammalian tissues that is involved in the energy supply of the parasite, were also detected.
在这项工作中，开展了二维凝胶电泳结合质谱分析，以着手构建克氏锥虫CL Brener株无鞭毛体形式的可溶性蛋白质图谱。该菌株是源自两个基因型（克氏锥虫I型和克氏锥虫II型）的杂交生物体，被选用于基因组测序。二维凝胶电泳显示，大多数蛋白质聚焦在pH 4 - 7范围内。鉴定表明，几种蛋白质存在多种同工型，如微管蛋白和热休克蛋白。还检测到了一些可作为化学治疗剂开发潜在靶点的蛋白质，如精氨酸激酶，这是一种在哺乳动物组织中不存在但参与寄生虫能量供应的酶。
