Noda Tomohiko, Kagoshima Kumiko, Uemori Akiko, Yasutake Koichi, Ichikawa Masayasu, Ohba Michio
Department of Applied Genetics and Pest Management, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan.
Curr Microbiol. 2009 Mar;58(3):195-200. doi: 10.1007/s00284-008-9307-5. Epub 2008 Nov 11.
A total of 39 Bacillus thuringiensis isolates were recovered from 38 leaves collected from 5- to 10-m-high canopies of 8 micro-/meso-phanerophyte species in a lucidophyllous forest of Japan. B. thuringiensis-positive leaves accounted for 1.4% of a total of 2805 leaves from 15 tree species. The frequency of the organism was 0.8% among the Bacillus cereus/B. thuringiensis group. Of 39 isolates obtained, 27 (69.2%) were allocated to 11 H serovars, and 12 isolates remained unidentified: 11 were motile but lacked reactivity to the 55 reference antisera, and 1 isolate was not flagellated. Two H serovars, kurstaki (H3abc) and tohokuensis (H17), occurred predominantly on canopy phylloplanes. Larvicidal activities against Bombyx mori and/or Aedes aegypti were associated with 49% of the canopy isolates. Strong hemolysis was induced by parasporal inclusion proteins of the two isolates of serovar israelensis (H14). Hemagglutinating (lectin) activity was associated with parasporal proteins of nine isolates. There was little correlation between insecticidal activity and lectin activity.
从日本常绿阔叶林8种小/中型显花植物5至10米高树冠采集的38片叶子中，共分离出39株苏云金芽孢杆菌。苏云金芽孢杆菌阳性叶片占15种树木2805片叶子总数的1.4%。该菌在蜡样芽孢杆菌/苏云金芽孢杆菌菌群中的出现频率为0.8%。在获得的39株分离株中，27株（69.2%）被归为11个H血清型，12株分离株未鉴定：11株有运动性，但对55种参考抗血清无反应，1株无鞭毛。两种H血清型，库斯塔克型（H3abc）和东北型（H17），主要出现在树冠叶面上。49%的树冠分离株对家蚕和/或埃及伊蚊有杀幼虫活性。血清型以色列型（H14）的两个分离株的伴孢晶体蛋白可诱导强烈溶血。9株分离株的伴孢蛋白具有血凝（凝集素）活性。杀虫活性与凝集素活性之间几乎没有相关性。
